Molecular characterization of canine parvovirus in Vientiane, Laos

The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82....

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Bibliographic Details
Published in:Archives of virology 2017-05, Vol.162 (5), p.1355-1361
Main Authors: Vannamahaxay, Soulasack, Vongkhamchanh, Souliya, Intanon, Montira, Tangtrongsup, Sahatchai, Tiwananthagorn, Saruda, Pringproa, Kidsadagon, Chuammitri, Phongsakorn
Format: Article
Language:English
Subjects:
Amino Acid Sequence - genetics
Animals
Base Sequence
Biomedical and Life Sciences
Biomedicine
Brief Report
Capsid Proteins - genetics
Disease Outbreaks - veterinary
DNA, Viral - genetics
Dog Diseases - epidemiology
Dog Diseases - virology
Dogs
Epidemics
Gastroenteritis - veterinary
Gastroenteritis - virology
Genes
Genetic Variation
Infectious Diseases
Laos - epidemiology
Maximum likelihood method
Medical Microbiology
Mutation
Parvoviridae
Parvoviridae Infections - epidemiology
Parvoviridae Infections - virology
Parvovirus, Canine - genetics
Parvovirus, Canine - isolation & purification
Phylogenetics
Phylogeny
Polymerase Chain Reaction
Proteins
Sequence Analysis, DNA
Veterinary medicine
Virology
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Summary:The global emergence of canine parvovirus type 2c (CPV-2c) has been well documented. In the present study, 139 rectal swab samples collected from diarrheic dogs living in Vientiane, Laos, in 2016 were tested for the presence of the canine parvovirus (CPV) VP2 gene by PCR. The results showed that 82.73% (115/139) of dogs were CPV positive by PCR. The partial VP2 gene was sequenced in 94 of the positive samples; 91 samples belonged to CPV-2c (426Glu) subtype, while 3 samples belonged to the CPV-2a (426Asn) subtype. Notably, phylogenetic analysis of amino acid sequences revealed a close relationship between Laotian isolates and novel Chinese CPV-2c isolates. In Laotian CPV isolates, aligned protein sequences indicated a high rate of residue substitutions at positions 305, 324, 345, 370, 375, and 426 in the GH loop. The mutation at residue 370 (Q370R), a single mutation, was characterized as a unique mutant residue specific to the Laotian CPV-2c variant.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-3212-1