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Lactococcus lactis KR‐050L inhibit IL‐6/STAT3 activation
Aims The purpose of this study was to investigate IL‐6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi. Methods and Results Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus...
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| Published in: | Journal of applied microbiology 2017-05, Vol.122 (5), p.1412-1422 |
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| container_end_page | 1422 |
| container_issue | 5 |
| container_start_page | 1412 |
| container_title | Journal of applied microbiology |
| container_volume | 122 |
| creator | Hwang, J.T. Jang, H.‐J. Kim, J.H. Park, C.S. Kim, Y. Lim, C.‐H. Lee, S.W. Rho, M.‐C. |
| description | Aims
The purpose of this study was to investigate IL‐6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi.
Methods and Results
Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR‐050L inhibited IL‐6‐induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative high‐performance liquid chromatography, and they were identified as 2,5‐diketopipperazine structures by spectroscopic analyses (MS, 1H‐ and 13C‐NMR). They also showed inhibitory activities on IL‐6‐induced STAT3 activation, and showed the different in activity according to the presence of a phenylalanine residue, hydroxyl groups and isometric structure.
Conclusions
The six new LABs isolated from Gajuknamu kimchi, and Lc. lactis KR‐050L was selected as candidate IL‐6/STAT3 inhibitors. The activity levels of 15 2,5‐DKPs isolated from Lc. lactis KR‐050L were verified.
Significance and Impact of the Study
This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL‐6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR‐050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL‐6/STAT3 activation. |
| doi_str_mv | 10.1111/jam.13444 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1891886724</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2058885715</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3864-6a08c55865cffb7eebd75ffef2dd2ba3d0d9fa6e9f1ede5113ab1670c21c41853</originalsourceid><addsrcrecordid>eNqNkM1KAzEUhYMoVqsLX0AG3Ohi2iSTZDLgphR_qiOC1nXIZBJMmZ866Sjd-Qg-o09i2qkuBMG7uZfLx7n3HACOEBwgX8OZLAcoIoRsgT0UMRpiFuPt9UxCCmPcA_vOzSBEEaRsF_QwxwkhjO6B81SqRa1qpVoXFH62Lrh9-Hz_gBSmga2ebWYXwST1GzZ8nI6mUbCCXuXC1tUB2DGycPpw0_vg6fJiOr4O0_uryXiUhiri_gMmIVeUckaVMVmsdZbH1BhtcJ7jTEY5zBMjmU4M0rmmCEUyQyyGCiNFEKdRH5x2uvOmfmm1W4jSOqWLQla6bp1APEGce8_kH2gcc0owZB49-YXO6rapvBGBIeWc0xitbp91lGpq5xptxLyxpWyWAkGxSl_49MU6fc8ebxTbrNT5D_kdtweGHfBmC738W0ncjO46yS-NYIz9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2058885715</pqid></control><display><type>article</type><title>Lactococcus lactis KR‐050L inhibit IL‐6/STAT3 activation</title><source>Alma/SFX Local Collection</source><creator>Hwang, J.T. ; Jang, H.‐J. ; Kim, J.H. ; Park, C.S. ; Kim, Y. ; Lim, C.‐H. ; Lee, S.W. ; Rho, M.‐C.</creator><creatorcontrib>Hwang, J.T. ; Jang, H.‐J. ; Kim, J.H. ; Park, C.S. ; Kim, Y. ; Lim, C.‐H. ; Lee, S.W. ; Rho, M.‐C.</creatorcontrib><description>Aims
The purpose of this study was to investigate IL‐6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi.
Methods and Results
Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR‐050L inhibited IL‐6‐induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative high‐performance liquid chromatography, and they were identified as 2,5‐diketopipperazine structures by spectroscopic analyses (MS, 1H‐ and 13C‐NMR). They also showed inhibitory activities on IL‐6‐induced STAT3 activation, and showed the different in activity according to the presence of a phenylalanine residue, hydroxyl groups and isometric structure.
Conclusions
The six new LABs isolated from Gajuknamu kimchi, and Lc. lactis KR‐050L was selected as candidate IL‐6/STAT3 inhibitors. The activity levels of 15 2,5‐DKPs isolated from Lc. lactis KR‐050L were verified.
Significance and Impact of the Study
This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL‐6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR‐050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL‐6/STAT3 activation.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13444</identifier><identifier>PMID: 28294465</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>2,5‐diketopipperazine ; Activation ; Biological Factors - chemistry ; Biological Factors - isolation & purification ; Biological Factors - pharmacology ; Brassica - microbiology ; Cell Line ; Chromatography ; Column chromatography ; Commercialization ; Culture ; Functional foods & nutraceuticals ; Gajuknamu kimchi ; Humans ; Hydroxyl groups ; IL‐6 ; Inhibitors ; Interleukin-6 - antagonists & inhibitors ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Isometric ; Kimchi ; Lactic acid ; Lactic acid bacteria ; Lactococcus lactis ; Lactococcus lactis - chemistry ; Lactococcus lactis - genetics ; Lactococcus lactis - isolation & purification ; Lactococcus lactis - metabolism ; Lactococcus lactis KR‐050L ; Liquid chromatography ; Medical treatment ; Molecular Structure ; NMR ; Nuclear magnetic resonance ; Phenylalanine ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; STAT3 ; Stat3 protein ; STAT3 Transcription Factor - antagonists & inhibitors ; STAT3 Transcription Factor - genetics ; STAT3 Transcription Factor - metabolism ; Vegetables - microbiology</subject><ispartof>Journal of applied microbiology, 2017-05, Vol.122 (5), p.1412-1422</ispartof><rights>2017 The Society for Applied Microbiology</rights><rights>2017 The Society for Applied Microbiology.</rights><rights>Copyright © 2017 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3864-6a08c55865cffb7eebd75ffef2dd2ba3d0d9fa6e9f1ede5113ab1670c21c41853</citedby><cites>FETCH-LOGICAL-c3864-6a08c55865cffb7eebd75ffef2dd2ba3d0d9fa6e9f1ede5113ab1670c21c41853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28294465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, J.T.</creatorcontrib><creatorcontrib>Jang, H.‐J.</creatorcontrib><creatorcontrib>Kim, J.H.</creatorcontrib><creatorcontrib>Park, C.S.</creatorcontrib><creatorcontrib>Kim, Y.</creatorcontrib><creatorcontrib>Lim, C.‐H.</creatorcontrib><creatorcontrib>Lee, S.W.</creatorcontrib><creatorcontrib>Rho, M.‐C.</creatorcontrib><title>Lactococcus lactis KR‐050L inhibit IL‐6/STAT3 activation</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
The purpose of this study was to investigate IL‐6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi.
Methods and Results
Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR‐050L inhibited IL‐6‐induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative high‐performance liquid chromatography, and they were identified as 2,5‐diketopipperazine structures by spectroscopic analyses (MS, 1H‐ and 13C‐NMR). They also showed inhibitory activities on IL‐6‐induced STAT3 activation, and showed the different in activity according to the presence of a phenylalanine residue, hydroxyl groups and isometric structure.
Conclusions
The six new LABs isolated from Gajuknamu kimchi, and Lc. lactis KR‐050L was selected as candidate IL‐6/STAT3 inhibitors. The activity levels of 15 2,5‐DKPs isolated from Lc. lactis KR‐050L were verified.
Significance and Impact of the Study
This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL‐6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR‐050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL‐6/STAT3 activation.</description><subject>2,5‐diketopipperazine</subject><subject>Activation</subject><subject>Biological Factors - chemistry</subject><subject>Biological Factors - isolation & purification</subject><subject>Biological Factors - pharmacology</subject><subject>Brassica - microbiology</subject><subject>Cell Line</subject><subject>Chromatography</subject><subject>Column chromatography</subject><subject>Commercialization</subject><subject>Culture</subject><subject>Functional foods & nutraceuticals</subject><subject>Gajuknamu kimchi</subject><subject>Humans</subject><subject>Hydroxyl groups</subject><subject>IL‐6</subject><subject>Inhibitors</subject><subject>Interleukin-6 - antagonists & inhibitors</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Isometric</subject><subject>Kimchi</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactococcus lactis</subject><subject>Lactococcus lactis - chemistry</subject><subject>Lactococcus lactis - genetics</subject><subject>Lactococcus lactis - isolation & purification</subject><subject>Lactococcus lactis - metabolism</subject><subject>Lactococcus lactis KR‐050L</subject><subject>Liquid chromatography</subject><subject>Medical treatment</subject><subject>Molecular Structure</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Phenylalanine</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>STAT3</subject><subject>Stat3 protein</subject><subject>STAT3 Transcription Factor - antagonists & inhibitors</subject><subject>STAT3 Transcription Factor - genetics</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Vegetables - microbiology</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqNkM1KAzEUhYMoVqsLX0AG3Ohi2iSTZDLgphR_qiOC1nXIZBJMmZ866Sjd-Qg-o09i2qkuBMG7uZfLx7n3HACOEBwgX8OZLAcoIoRsgT0UMRpiFuPt9UxCCmPcA_vOzSBEEaRsF_QwxwkhjO6B81SqRa1qpVoXFH62Lrh9-Hz_gBSmga2ebWYXwST1GzZ8nI6mUbCCXuXC1tUB2DGycPpw0_vg6fJiOr4O0_uryXiUhiri_gMmIVeUckaVMVmsdZbH1BhtcJ7jTEY5zBMjmU4M0rmmCEUyQyyGCiNFEKdRH5x2uvOmfmm1W4jSOqWLQla6bp1APEGce8_kH2gcc0owZB49-YXO6rapvBGBIeWc0xitbp91lGpq5xptxLyxpWyWAkGxSl_49MU6fc8ebxTbrNT5D_kdtweGHfBmC738W0ncjO46yS-NYIz9</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Hwang, J.T.</creator><creator>Jang, H.‐J.</creator><creator>Kim, J.H.</creator><creator>Park, C.S.</creator><creator>Kim, Y.</creator><creator>Lim, C.‐H.</creator><creator>Lee, S.W.</creator><creator>Rho, M.‐C.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201705</creationdate><title>Lactococcus lactis KR‐050L inhibit IL‐6/STAT3 activation</title><author>Hwang, J.T. ; Jang, H.‐J. ; Kim, J.H. ; Park, C.S. ; Kim, Y. ; Lim, C.‐H. ; Lee, S.W. ; Rho, M.‐C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3864-6a08c55865cffb7eebd75ffef2dd2ba3d0d9fa6e9f1ede5113ab1670c21c41853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>2,5‐diketopipperazine</topic><topic>Activation</topic><topic>Biological Factors - chemistry</topic><topic>Biological Factors - isolation & purification</topic><topic>Biological Factors - pharmacology</topic><topic>Brassica - microbiology</topic><topic>Cell Line</topic><topic>Chromatography</topic><topic>Column chromatography</topic><topic>Commercialization</topic><topic>Culture</topic><topic>Functional foods & nutraceuticals</topic><topic>Gajuknamu kimchi</topic><topic>Humans</topic><topic>Hydroxyl groups</topic><topic>IL‐6</topic><topic>Inhibitors</topic><topic>Interleukin-6 - antagonists & inhibitors</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Isometric</topic><topic>Kimchi</topic><topic>Lactic acid</topic><topic>Lactic acid bacteria</topic><topic>Lactococcus lactis</topic><topic>Lactococcus lactis - chemistry</topic><topic>Lactococcus lactis - genetics</topic><topic>Lactococcus lactis - isolation & purification</topic><topic>Lactococcus lactis - metabolism</topic><topic>Lactococcus lactis KR‐050L</topic><topic>Liquid chromatography</topic><topic>Medical treatment</topic><topic>Molecular Structure</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Phenylalanine</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>STAT3</topic><topic>Stat3 protein</topic><topic>STAT3 Transcription Factor - antagonists & inhibitors</topic><topic>STAT3 Transcription Factor - genetics</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Vegetables - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, J.T.</creatorcontrib><creatorcontrib>Jang, H.‐J.</creatorcontrib><creatorcontrib>Kim, J.H.</creatorcontrib><creatorcontrib>Park, C.S.</creatorcontrib><creatorcontrib>Kim, Y.</creatorcontrib><creatorcontrib>Lim, C.‐H.</creatorcontrib><creatorcontrib>Lee, S.W.</creatorcontrib><creatorcontrib>Rho, M.‐C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, J.T.</au><au>Jang, H.‐J.</au><au>Kim, J.H.</au><au>Park, C.S.</au><au>Kim, Y.</au><au>Lim, C.‐H.</au><au>Lee, S.W.</au><au>Rho, M.‐C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactococcus lactis KR‐050L inhibit IL‐6/STAT3 activation</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2017-05</date><risdate>2017</risdate><volume>122</volume><issue>5</issue><spage>1412</spage><epage>1422</epage><pages>1412-1422</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
The purpose of this study was to investigate IL‐6/STAT3 inhibitory activity using lactic acid bacteria (LABs) isolated from Gajuknamu kimchi.
Methods and Results
Six LABs were isolated from Gajuknamu kimchi and identified through 16S rRNA sequencing. Among them, the culture broth of Lactococcus lactis KR‐050L inhibited IL‐6‐induced STAT3 luciferase activity. Fifteen compounds were isolated from the EtOAc extract of culture broth though column chromatography and preparative high‐performance liquid chromatography, and they were identified as 2,5‐diketopipperazine structures by spectroscopic analyses (MS, 1H‐ and 13C‐NMR). They also showed inhibitory activities on IL‐6‐induced STAT3 activation, and showed the different in activity according to the presence of a phenylalanine residue, hydroxyl groups and isometric structure.
Conclusions
The six new LABs isolated from Gajuknamu kimchi, and Lc. lactis KR‐050L was selected as candidate IL‐6/STAT3 inhibitors. The activity levels of 15 2,5‐DKPs isolated from Lc. lactis KR‐050L were verified.
Significance and Impact of the Study
This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL‐6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR‐050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL‐6/STAT3 activation.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>28294465</pmid><doi>10.1111/jam.13444</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 1364-5072 |
| ispartof | Journal of applied microbiology, 2017-05, Vol.122 (5), p.1412-1422 |
| issn | 1364-5072 1365-2672 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | 2,5‐diketopipperazine Activation Biological Factors - chemistry Biological Factors - isolation & purification Biological Factors - pharmacology Brassica - microbiology Cell Line Chromatography Column chromatography Commercialization Culture Functional foods & nutraceuticals Gajuknamu kimchi Humans Hydroxyl groups IL‐6 Inhibitors Interleukin-6 - antagonists & inhibitors Interleukin-6 - genetics Interleukin-6 - metabolism Isometric Kimchi Lactic acid Lactic acid bacteria Lactococcus lactis Lactococcus lactis - chemistry Lactococcus lactis - genetics Lactococcus lactis - isolation & purification Lactococcus lactis - metabolism Lactococcus lactis KR‐050L Liquid chromatography Medical treatment Molecular Structure NMR Nuclear magnetic resonance Phenylalanine RNA, Ribosomal, 16S - genetics rRNA 16S STAT3 Stat3 protein STAT3 Transcription Factor - antagonists & inhibitors STAT3 Transcription Factor - genetics STAT3 Transcription Factor - metabolism Vegetables - microbiology |
| title | Lactococcus lactis KR‐050L inhibit IL‐6/STAT3 activation |
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