Application of gold nanoparticle-assisted PCR for equine herpesvirus 1 diagnosis in field samples

Equine herpesvirus 1 (EHV-1) is one of the most significant pathogens that affects equine species worldwide, causing sporadic abortion, neonatal deaths, chorioretinopathy, as well as neurological and upper respiratory tract diseases. Currently, conventional PCR targeting different genes is used wide...

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Bibliographic Details
Published in:Archives of virology 2017-08, Vol.162 (8), p.2297-2303
Main Authors: El-Husseini, Dalia M., Helmy, Nashwa M., Tammam, Reham H.
Format: Article
Language:English
Subjects:
Abortion
Animals
Biomedical and Life Sciences
Biomedicine
Cross-reactivity
Diagnosis
DNA, Viral - isolation & purification
Fish
Glycoprotein B
Gold
Herpesviridae Infections - diagnosis
Herpesviridae Infections - veterinary
Herpesvirus 1, Equid - isolation & purification
Horse Diseases - diagnosis
Horse Diseases - virology
Horses - virology
Infectious Diseases
Medical Microbiology
Nanoparticles
Neonates
Original Article
Polymerase chain reaction
Real-Time Polymerase Chain Reaction - methods
Real-Time Polymerase Chain Reaction - veterinary
Respiratory tract diseases
Sensitivity and Specificity
Virology
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Summary:Equine herpesvirus 1 (EHV-1) is one of the most significant pathogens that affects equine species worldwide, causing sporadic abortion, neonatal deaths, chorioretinopathy, as well as neurological and upper respiratory tract diseases. Currently, conventional PCR targeting different genes is used widely for the molecular detection of EHV-1, but the low viral titer in some clinical samples can lead to false negative results. In this study, we aimed to assess gold nanoparticle (GNP)-assisted PCR as an inexpensive, highly efficient, and sensitive method for the detection of EHV-1, and to compare its results with conventional PCR and real-time quantitative PCR (qPCR). Out of 83 field samples, 28.9%, 26.5%, and 15.6% were EHV-1-positive by qPCR, GNP-assisted PCR and conventional PCR, respectively. All three techniques specifically target the viral glycoprotein B gene. The optimized GNP-assisted PCR showed no cross-reactivity with EHV-1-negative samples (diagnosed by qPCR). GNP-assisted PCR is a powerful new tool for EHV-1 detection and surveillance, because of its simplicity, sensitivity and specificity. It can be used as an alternative to qPCR in laboratories that cannot afford the expense of a qPCR system.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-017-3379-0