Dietary mannoheptulose does not alter glucose or lipid metabolism in adult Labrador Retrievers

• Published in: Journal of animal physiology and animal nutrition 2018-02, Vol.102 (1), p.e122-e131
• Main Authors: McKnight, L. L., France, J., Wright, D., Davenport, G., Shoveller, A. K.
• Format: Article
• Language: English
• Subjects: acetate; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animals; Blood Glucose; Calorimetry; canine; Cross-Over Studies; Diet; Diet - veterinary; Dogs; Double-Blind Method; Fasting; Fatty acids; Feeding; free fatty acid; Functional foods & nutraceuticals; Glucose; Glucose - metabolism; Glycerol; Glycolysis; Heat measurement; insulin; Kinetics; Laboratory testing; Lipid metabolism; Lipid Metabolism - drug effects; Lipolysis; Mannoheptulose - administration & dosage; Mannoheptulose - pharmacology; Meal feeding; Metabolism; Oxidation; Palmitic acid; rate of appearance; Tracers
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.12713

Summary Mannoheptulose (MH), a glycolytic inhibitor, has been preliminarily investigated as a novel functional food ingredient for dogs. This study aimed to determine the effects of dietary MH, delivered as an extract of un‐ripened avocados, on fatty acid and glucose kinetics in healthy adult Labrador Retriever dogs (n = 12 dogs). The study was a double‐blindcrossover with each dog receiving both dietary treatments, control (CON) and MH (400 mg/kg of diet), in random order. Glucose and glycerol plasma turnover (Ra) and oxidation (Ox) were measured in fasting and in response to repeated meal feeding (“fed”) with stable isotope tracers (U‐13C‐glucose, 1,1,2,3,3‐D5‐glycerol) and indirect calorimetry. Palmitate Ra and Ox were examined during repeated meal feeding only using an oral bolus of U‐13C‐K2‐palmitate and indirect calorimetry. MH had no discernible effect on fasting glucose Ra (677, 722 SEM 36 μmol/min, CON, MH) or Ox (107, 109 μmol/min, CON, MH SEM 10 μmol/min) or fed glucose Ra (2913, 3626 SEM 644 μmol/min, CON, MH) or Ox (951, 936 SEM 174 μmol/min, CON, MH). Glycerol Ra, an index of the rate of lipolysis, was not different between dietary treatments (Fast 162, 113 SEM 35 μmol/min CON, MH; Fed 172, 135 SEM 21 μmol/min, CON, MH). Similarly, palmitate oxidation was not impacted by MH feeding (1966, 2276 SEM 79 μmol/min, CON, MH). Together, these findings do not support MH as a novel functional food ingredient at least at the dietary dose tested.