Growth engineering of Synechococcus elongatus PCC 7942 for mixotrophy under natural light conditions for improved feedstock production

• Published in: Biotechnology progress 2017-09, Vol.33 (5), p.1182-1192
• Main Authors: Sarnaik, Aditya, Pandit, Reena, Lali, Arvind
• Format: Article
• Language: English
• Subjects: Autotrophs; Autotrophy; Biomass; Bioreactors - microbiology; Carotenoids; Carotenoids - analysis; Carotenoids - metabolism; Chlorophyll; Chlorophyll - analysis; Chlorophyll - metabolism; Cloning, Molecular; Elongation; Energy metabolism; Environmental chambers; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; galP; Genetic engineering; Hexose; Hexose transporter; Light; Metabolic Engineering - methods; Metabolism; Mixotrophy; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; natural diurnal light; Photobioreactors; Productivity; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Sunlight; Synechococcus; Synechococcus - genetics; Synechococcus - metabolism; Synechococcus - physiology; Synechococcus elongatus; Temperature control; Transportation services; Zeaxanthin
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1002/btpr.2490

Synechococcus elongatus PCC 7942 has been widely explored as cyanobacterial cell factory through genetic modifications for production of various value‐added compounds. However, successful industrial scale‐ups have not been reported for the system predominantly due to its obligate photoautotrophic metabolism and use of artificial light in photobioreactors. Hence, engineering the organism to perform mixotrophy under natural light could serve as an effective solution. Thus, we applied a genetically engineered strain of Synechococcus elongatus PCC 7942 expressing heterologous hexose transporter gene (galP) to perform mixotrophy under natural light in a temperature controlled environmental chamber (EC). We systematically studied the comparative performances of these transformants using autotrophy and mixotrophy, which showed 3.4 times increase in biomass productivity of mixotrophically grown transformants over autotrophs in EC. Chlorophyll a yield was found to have decreased in mixotrophic conditions, possibly indicating reduced dependency on light for energy metabolism. Although pigment yield decreases under mixotrophy, titer was found to have improved due to increased biomass productivity. Carotenoid analysis showed that zeaxanthin is the major carotenoid produced by the species which is essential for photoprotection. Our work thus demonstrates that mixotrophy under temperature controlled natural light can serve as the viable solution to improve biomass productivity of Synechococcus elongatus PCC 7942 and for commercial production of natural or engineered value added compounds from the system. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1182–1192, 2017