Monitoring the cellular activity of a cultured single cell by scanning electrochemical microscopy (SECM). A comparison with fluorescence viability monitoring

The respiratory activities of cultured HeLa cells were monitored at a single cell level using scanning electrochemical microscopy (SECM) that produces images of the localized distribution of oxygen around the cell. The change in the cellular activity was traced after exposures to KCN, ethyl alcohol...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2003-10, Vol.18 (11), p.1379-1383
Main Authors: Kaya, Takatoshi, Torisawa, Yu-suke, Oyamatsu, Daisuke, Nishizawa, Matsuhiko, Matsue, Tomokazu
Format: Article
Language:English
Subjects:
antimycin A
Antimycin A - pharmacology
Biological and medical sciences
Biotechnology
Calcein
Cell Culture Techniques - methods
Cell Respiration - drug effects
Cell Respiration - physiology
Cytotoxicity
Electrochemistry - methods
Ethanol - pharmacology
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Methods. Procedures. Technologies
Microelectrodes
Microscopy, Confocal - methods
Others
Oxygen - metabolism
potassium cyanide
Potassium Cyanide - pharmacology
Respiration activity
Scanning electrochemical microscopy (SECM)
Spectrometry, Fluorescence - methods
Various methods and equipments
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Summary:The respiratory activities of cultured HeLa cells were monitored at a single cell level using scanning electrochemical microscopy (SECM) that produces images of the localized distribution of oxygen around the cell. The change in the cellular activity was traced after exposures to KCN, ethyl alcohol and the antibiotic drug, Antimycin A. The results were compared with those from the conventional fluorescence monitoring using Calcein-AM that is sensitive to deformation of the cell membrane. The SECM-based measurement follows the decrease in the cellular activity upon exposure to KCN and Antimycin A more rapidly than the fluorescence-based measurements, demonstrating that SECM is suitable for studying the cellular influence of respiration inhibitors.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(03)00083-6