Mucoadhesive properties of low molecular weight chitosan- or glycol chitosan- and corresponding thiomer-coated poly(isobutylcyanoacrylate) core-shell nanoparticles

• Published in: European journal of pharmaceutics and biopharmaceutics 2017-08, Vol.117, p.315-323
• Main Authors: Palazzo, Claudio, Trapani, Giuseppe, Ponchel, Gilles, Trapani, Adriana, Vauthier, Christine
• Format: Article
• Language: English
• Subjects: Animals; Chitosan; Chitosan - chemistry; Chitosan - metabolism; Cyanoacrylates - chemistry; Cyanoacrylates - metabolism; Drug Carriers - chemistry; Drug Carriers - metabolism; Glycol chitosan; Intestinal Mucosa - metabolism; Jejunum - metabolism; Male; Molecular Weight; Mucoadhesion; Nanoparticles - chemistry; Nanoparticles - metabolism; Organ Culture Techniques; Rats; Rats, Wistar; Thiomers; Ussing chambers
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2017.04.020

[Display omitted] The aim of the present work was to evaluate the mucoadhesive properties of poly(isobutyl cyanoacrylate) (PIBCA) nanoparticles (NPs) coated with Low Molecular Weight (LMW) chitosan (CS)- and glycol chitosan (GCS)-based thiomers as well as with the corresponding LMW unmodified polysaccharides. For this purpose, all the CS- and GCS-based thiomers were prepared under simple and mild conditions starting from the LMW unmodified polymers CS and GCS. The resulting NPs were of spherical shape with diameters ranging from 400 to 600nm and 187 to 309nm, for CS- and GCS-based NPs, respectively. The mucoadhesive characteristics of these core shell NPs were studied in Ussing chambers measuring the percentage of NPs stuck on the mucosal of fresh intestinal tissue after 2h of incubation. Moreover, incubation of nanoparticle formulations with the intestinal tissue induced changes in transmucosal electrical resistance which were measured to gain information into the opening of tight junctions and to control the integrity of the mucosa. Thus, it was found that PIBCA NPs coated with the GCS–Glutathione conjugate (GCGPIBCA NPs) possessed the most favorable mucoadhesive performances. Moreover, both GCGPIBCA- and GCS-N-acetyl-cysteine (GCNPIBCA)-core-shell NPs might induced an enlargement of the epithelial cell tight junctions. In conclusion, coating of PIBCA NPs with GCS-based thiomers may be useful for improving the mucoadhesive and permeation properties of these nanocarriers.