Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods

Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2003-03, Vol.39 (2), p.236-239
Main Authors: Rosa María Escobedo Gracia-Medrano, María de Lourdes Miranda-Ham
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell culture techniques
Cell death
Cell lines
Cell membranes
Chemical suspensions
Cultured cells
Fundamental and applied biological sciences. Psychology
Hypersensitive response
Lycopersicon esculentum
Pathogens
Plant cells
Plant physiology and development
Research Reports
Tissue cultures, protoplasts
Viability
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Summary:Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated as an early elicitor-induced viability change in suspension cultures, it is strongly advisable to use the assay based on the reduction of tetrazolium salts, since it provides an adequate indicator of changes in metabolic activity that could otherwise be overlooked.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2002395