Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods

Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2003-03, Vol.39 (2), p.236-239
Main Authors: Rosa María Escobedo Gracia-Medrano, María de Lourdes Miranda-Ham
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell culture techniques
Cell death
Cell lines
Cell membranes
Chemical suspensions
Cultured cells
Fundamental and applied biological sciences. Psychology
Hypersensitive response
Lycopersicon esculentum
Pathogens
Plant cells
Plant physiology and development
Research Reports
Tissue cultures, protoplasts
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83
cites cdi_FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83
container_end_page 239
container_issue 2
container_start_page 236
container_title In vitro cellular & developmental biology. Plant
container_volume 39
creator Rosa María Escobedo Gracia-Medrano
María de Lourdes Miranda-Ham
description Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated as an early elicitor-induced viability change in suspension cultures, it is strongly advisable to use the assay based on the reduction of tetrazolium salts, since it provides an adequate indicator of changes in metabolic activity that could otherwise be overlooked.
doi_str_mv 10.1079/IVP2002395
format article
fullrecord <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_18936648</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4293601</jstor_id><sourcerecordid>4293601</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83</originalsourceid><addsrcrecordid>eNpd0UFrFDEUB_BBFKzVi2cPQdCDMDWZzEySY5lWXdiioPY6ZJMXmyWbrHmTw4Af3pQtFjwl8H55Sd6_aV4zesGoUB83t986SjuuhifNGevF0HajVE_rng59O_RifN68QNxTShll4qz5cxl1WNEjSY5cB2_8knK7ibYYsGSCEMit1zsf_LKS6U7HX4DER7JdTTpCRm9SJICmBIhLOZAbH8IF-V7wCBF9rU0lLCUD2a3kyjsHuTpyA8tdsviyeeZ0QHj1sJ43Pz9d_5i-tNuvnzfT5bY1nMultQM4pqm0VrGxk4MxtgfHB0WdGBnTo-EMKAPhxq5CKwfbKcGgYs7kTvLz5v2p7zGn3wVwmQ8eTf2bjpAKzkwqPo79PXz7H9ynkuuEqlGCqzozXtGHEzI5IWZw8zH7g87rzOh8n8L8mELF7x46ajQ6uKyj8fh4oheq41JU9-bk9lgD-Ffvu_qyeulf2JiQ2Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>197391013</pqid></control><display><type>article</type><title>Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods</title><source>Springer Nature</source><source>JSTOR</source><creator>Rosa María Escobedo Gracia-Medrano ; María de Lourdes Miranda-Ham</creator><creatorcontrib>Rosa María Escobedo Gracia-Medrano ; María de Lourdes Miranda-Ham</creatorcontrib><description>Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated as an early elicitor-induced viability change in suspension cultures, it is strongly advisable to use the assay based on the reduction of tetrazolium salts, since it provides an adequate indicator of changes in metabolic activity that could otherwise be overlooked.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1079/IVP2002395</identifier><language>eng</language><publisher>Wallingford: CABI Publishing</publisher><subject>Biological and medical sciences ; Cell culture techniques ; Cell death ; Cell lines ; Cell membranes ; Chemical suspensions ; Cultured cells ; Fundamental and applied biological sciences. Psychology ; Hypersensitive response ; Lycopersicon esculentum ; Pathogens ; Plant cells ; Plant physiology and development ; Research Reports ; Tissue cultures, protoplasts ; Viability</subject><ispartof>In vitro cellular &amp; developmental biology. Plant, 2003-03, Vol.39 (2), p.236-239</ispartof><rights>Copyright 2003 Society for In Vitro Biology</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Society for In Vitro Biology Mar/Apr 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83</citedby><cites>FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4293601$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4293601$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=14792387$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosa María Escobedo Gracia-Medrano</creatorcontrib><creatorcontrib>María de Lourdes Miranda-Ham</creatorcontrib><title>Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods</title><title>In vitro cellular &amp; developmental biology. Plant</title><description>Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated as an early elicitor-induced viability change in suspension cultures, it is strongly advisable to use the assay based on the reduction of tetrazolium salts, since it provides an adequate indicator of changes in metabolic activity that could otherwise be overlooked.</description><subject>Biological and medical sciences</subject><subject>Cell culture techniques</subject><subject>Cell death</subject><subject>Cell lines</subject><subject>Cell membranes</subject><subject>Chemical suspensions</subject><subject>Cultured cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypersensitive response</subject><subject>Lycopersicon esculentum</subject><subject>Pathogens</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Research Reports</subject><subject>Tissue cultures, protoplasts</subject><subject>Viability</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpd0UFrFDEUB_BBFKzVi2cPQdCDMDWZzEySY5lWXdiioPY6ZJMXmyWbrHmTw4Af3pQtFjwl8H55Sd6_aV4zesGoUB83t986SjuuhifNGevF0HajVE_rng59O_RifN68QNxTShll4qz5cxl1WNEjSY5cB2_8knK7ibYYsGSCEMit1zsf_LKS6U7HX4DER7JdTTpCRm9SJICmBIhLOZAbH8IF-V7wCBF9rU0lLCUD2a3kyjsHuTpyA8tdsviyeeZ0QHj1sJ43Pz9d_5i-tNuvnzfT5bY1nMultQM4pqm0VrGxk4MxtgfHB0WdGBnTo-EMKAPhxq5CKwfbKcGgYs7kTvLz5v2p7zGn3wVwmQ8eTf2bjpAKzkwqPo79PXz7H9ynkuuEqlGCqzozXtGHEzI5IWZw8zH7g87rzOh8n8L8mELF7x46ajQ6uKyj8fh4oheq41JU9-bk9lgD-Ffvu_qyeulf2JiQ2Q</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Rosa María Escobedo Gracia-Medrano</creator><creator>María de Lourdes Miranda-Ham</creator><general>CABI Publishing</general><general>Cambridge University Press</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20030301</creationdate><title>Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods</title><author>Rosa María Escobedo Gracia-Medrano ; María de Lourdes Miranda-Ham</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Cell culture techniques</topic><topic>Cell death</topic><topic>Cell lines</topic><topic>Cell membranes</topic><topic>Chemical suspensions</topic><topic>Cultured cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypersensitive response</topic><topic>Lycopersicon esculentum</topic><topic>Pathogens</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Research Reports</topic><topic>Tissue cultures, protoplasts</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosa María Escobedo Gracia-Medrano</creatorcontrib><creatorcontrib>María de Lourdes Miranda-Ham</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular &amp; developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosa María Escobedo Gracia-Medrano</au><au>María de Lourdes Miranda-Ham</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods</atitle><jtitle>In vitro cellular &amp; developmental biology. Plant</jtitle><date>2003-03-01</date><risdate>2003</risdate><volume>39</volume><issue>2</issue><spage>236</spage><epage>239</epage><pages>236-239</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>Viability changes and hydrogen peroxide production in cultured Lycopersicon esculentum cv. Rio Grande (tomato) cells exposed to induced stress conditions have been investigated. Our data suggest that in order to obtain reliable and insightful data when the hypersensitive response is being evaluated as an early elicitor-induced viability change in suspension cultures, it is strongly advisable to use the assay based on the reduction of tetrazolium salts, since it provides an adequate indicator of changes in metabolic activity that could otherwise be overlooked.</abstract><cop>Wallingford</cop><pub>CABI Publishing</pub><doi>10.1079/IVP2002395</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1054-5476
ispartof In vitro cellular & developmental biology. Plant, 2003-03, Vol.39 (2), p.236-239
issn 1054-5476
1475-2689
language eng
recordid cdi_proquest_miscellaneous_18936648
source Springer Nature; JSTOR
subjects Biological and medical sciences
Cell culture techniques
Cell death
Cell lines
Cell membranes
Chemical suspensions
Cultured cells
Fundamental and applied biological sciences. Psychology
Hypersensitive response
Lycopersicon esculentum
Pathogens
Plant cells
Plant physiology and development
Research Reports
Tissue cultures, protoplasts
Viability
title Analysis of Elicitor-Induced Cell Viability Changes in Lycopersicon esculentum Mill. Suspension Culture by Different Methods
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T09%3A48%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20Elicitor-Induced%20Cell%20Viability%20Changes%20in%20Lycopersicon%20esculentum%20Mill.%20Suspension%20Culture%20by%20Different%20Methods&rft.jtitle=In%20vitro%20cellular%20&%20developmental%20biology.%20Plant&rft.au=Rosa%20Mar%C3%ADa%20Escobedo%20Gracia-Medrano&rft.date=2003-03-01&rft.volume=39&rft.issue=2&rft.spage=236&rft.epage=239&rft.pages=236-239&rft.issn=1054-5476&rft.eissn=1475-2689&rft_id=info:doi/10.1079/IVP2002395&rft_dat=%3Cjstor_proqu%3E4293601%3C/jstor_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c338t-d5ef1a08dd916285ccd4ef3590f7611a6c31e01e7f62f1ad85d2971e162318b83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=197391013&rft_id=info:pmid/&rft_jstor_id=4293601&rfr_iscdi=true