Luminescence-based whole-cell-sensing systems for cadmium and lead using genetically engineered bacteria

Whole-cell-based sensing systems that respond to cadmium and lead ions have been designed and developed using genetically engineered bacteria. These systems take advantage of the ability of certain bacteria to survive in environments polluted with cadmium and lead ions. The bacteria used in this inv...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2003-05, Vol.376 (1), p.11-17
Main Authors: Shetty, Ranjit S, Deo, Sapna K, Shah, Puja, Sun, Yan, Rosen, Barry P, Daunert, Sylvia
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
CadC protein
cadmium
Cadmium - analysis
Cadmium - chemistry
Cadmium - pharmacology
cobalt
copper
Dose-Response Relationship, Drug
Escherichia coli - chemistry
Escherichia coli - drug effects
Escherichia coli - genetics
Genetic Engineering
green fluorescent protein
lead
Lead - analysis
Lead - chemistry
Lead - pharmacology
Luminescent Measurements
manganese
nickel
plasmids
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Summary:Whole-cell-based sensing systems that respond to cadmium and lead ions have been designed and developed using genetically engineered bacteria. These systems take advantage of the ability of certain bacteria to survive in environments polluted with cadmium and lead ions. The bacteria used in this investigation have been genetically engineered to produce reporter proteins in response to the toxic ions. This was achieved by modifying a strain of Escherichia colito harbor plasmids pYSC1 and pYS2/pYSG1. In these dual-plasmid-based sensing systems, the expression of the reporters beta-galactosidase and red-shifted green fluorescent protein (rs-GFP) was controlled by CadC, the regulatory protein of the cad operon. Regulation of the expression of the reporter proteins is related to the amount of cadmium and lead ions employed to induce the bacteria. The bacterial sensing systems were found to respond to cadmium, lead, and zinc ions, and had no significant response to nickel, copper, manganese, and cobalt.
ISSN:1618-2642
DOI:10.1007/s00216-003-1862-9