A bioorthogonal super(68)Ga-labelling strategy for rapid in vivo imaging

Herein, we describe a fast and robust method for achieving super(68)Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels-Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the meth...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-07, Vol.50 (67), p.9557-9560
Main Authors: Evans, Helen L, Nguyen, Quang-De, Carroll, Laurence S, Kaliszczak, Maciej, Twyman, Frazer J, Spivey, Alan C, Aboagye, Eric O
Format: Article
Language:English
Subjects:
Monoclonal antibodies
Robustness
Strategy
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Summary:Herein, we describe a fast and robust method for achieving super(68)Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels-Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc03903c