Photobiomodulation laser and pulsed electrical field increase the viability of the musculocutaneous flap in diabetic rats

The purpose of this study is to investigate the effect of pulsed electrical field (PEF) and photobiomodulation laser (PBM) on the viability of the TRAM flap in diabetic rats. Fifty Wistar rats were divided into five homogeneous groups: Group 1—control; Group 2—diabetics; Group 3—diabetics + PEF; Gro...

Full description

Saved in:
Bibliographic Details
Published in:Lasers in medical science 2017-04, Vol.32 (3), p.641-648
Main Authors: Leite, Gabriella de Paula Marcondes Ferreira, das Neves, Lais Mara Siqueira, Silva, Carlos Alberto, Guirro, Rinaldo Roberto de Jesus, de Souza, Thamires Rodrigues, de Souza, Ana Karina, Garcia, Sérgio Britto, Guirro, Elaine Caldeira de Oliveira
Format: Article
Language:English
Subjects:
Animals
Blood vessels
Cell Survival - radiation effects
Dentistry
Diabetes
Diabetes Mellitus, Experimental - pathology
Diabetes Mellitus, Experimental - radiotherapy
Electric fields
Electricity
Epidermis
Fibroblast Growth Factors - metabolism
Flaps
Lasers
Leukocytes
Leukocytes - pathology
Leukocytes - radiation effects
Low-Level Light Therapy
Male
Mast Cells - metabolism
Mast Cells - pathology
Mast Cells - radiation effects
Masts
Medicine
Medicine & Public Health
Myocutaneous Flap - pathology
Necrosis
Optical Devices
Optics
Original Article
Photonics
Quantum Optics
Rats
Rats, Wistar
Rodents
Skin Temperature - radiation effects
Surgical outcomes
Vascular Endothelial Growth Factor A - metabolism
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The purpose of this study is to investigate the effect of pulsed electrical field (PEF) and photobiomodulation laser (PBM) on the viability of the TRAM flap in diabetic rats. Fifty Wistar rats were divided into five homogeneous groups: Group 1—control; Group 2—diabetics; Group 3—diabetics + PEF; Group 4—diabetic + laser 660 nm, 10 J/cm 2 , 0.27 J; Group 5—diabetic + laser 660 nm, 140 J/cm 2 , 3.9 J. The percentage of necrotic area was evaluated using software Image J®. The peripheral circulation of the flap was evaluated by infrared thermography FLIR T450sc (FLIR® Systems—Oregon USA). The thickness of the epidermis (haematoxylin-eosin), mast cell (toluidine blue), leukocytes, vascular endothelial growth factor, fibroblast and newly formed blood vessels were evaluated. For the statistical analysis, the Kruskal-Wallis test was applied followed by Dunn and ANOVA test followed by Tukey with critical level of 5% ( p  
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-017-2160-7