Occurrence of erythromycin and its degradation products residues in honey. Validation of an analytical method

Erythromycin A, the main component of erythromycin, is widely used to treat and control foulbrood diseases in honey bees. In this study, we developed a fast and sensitive method to simultaneously determine erythromycin A and its degradation products in honey. The analytical methodology was based on...

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Bibliographic Details
Published in:Journal of separation science 2017-03, Vol.40 (6), p.1353-1360
Main Authors: Zhao, Liuwei, Cao, Weirui, Xue, Xiaofeng, Wang, Miao, Wu, Liming, Yu, Linsheng
Format: Article
Language:English
Subjects:
Analytical chemistry
Animal diseases
Animals
Antibiotics
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Liquid
Degradation
degradation products
Diseases
Drug Residues - isolation & purification
Erythromycin
Erythromycin - isolation & purification
Food Contamination - analysis
Honey
Honey - analysis
Liquid chromatography
liquid chromatography with tandem mass spectrometry
liquid–liquid microextraction
Mass spectrometry
Mathematical analysis
Reproducibility of Results
Tandem Mass Spectrometry
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Summary:Erythromycin A, the main component of erythromycin, is widely used to treat and control foulbrood diseases in honey bees. In this study, we developed a fast and sensitive method to simultaneously determine erythromycin A and its degradation products in honey. The analytical methodology was based on dispersive liquid–liquid microextraction and liquid chromatography coupled with tandem mass spectrometry with advanced i‐Funnel technology. The liquid–liquid microextraction and liquid chromatography coupled with tandem mass spectrometry parameters were optimized. The recoveries of erythromycin A and its degradation products from spiked honey samples were 76.1–102.1%, with reproducibility rates of 7.1–13.1% and correlation coefficients  >0.99. The decision limit and detection capability were 0.02–0.07 and 0.03–0.10 ng/g, respectively. The proposed method was validated and successfully applied to the determination of the target analytes in commercial honey samples. It was efficient and sensitive, and it lays the foundation for further research on honey safety.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201601257