One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1

An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation. Synthetic Mambalgin-1 exhibited a well-defined structure after sequential folding in vitro. NMR spectroscopy revealed a three-f...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-01, Vol.50 (44), p.5837-5839
Main Authors: Pan, Man, He, Yao, Wen, Ming, Wu, Fangming, Sun, Demeng, Li, Sijian, Zhang, Longhua, Li, Yiming, Tian, Changlin
Format: Article
Language:English
Subjects:
Acid Sensing Ion Channels - drug effects
Azides - chemistry
Elapid Venoms - chemical synthesis
Elapid Venoms - chemistry
Elapid Venoms - toxicity
Folding
Inhibition
Ion channels
Models, Molecular
NMR spectroscopy
Nuclear Magnetic Resonance, Biomolecular
Peptides - chemical synthesis
Peptides - chemistry
Peptides - toxicity
Protein Conformation
Strategy
Synthesis (chemistry)
Toxins
Venom toxins
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Summary:An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation. Synthetic Mambalgin-1 exhibited a well-defined structure after sequential folding in vitro. NMR spectroscopy revealed a three-finger toxin family structure, and the synthetic toxin inhibited human acid-sensing ion channel 1a.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc00779d