Transient expression of recombinant tissue plasminogen activator (rt-PA) gene in cucurbit plants using viral vector

Objective To use a transient expression system to express a truncated human tissue plasminogen activator (K2S) gene in cucurbit plants. Results The recombinant tissue plasminogen activator protein (K2S form) was expressed in active form in cucurbit plants. Its molecular weight was 43 kDa. The plant-...

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Bibliographic Details
Published in:Biotechnology letters 2017-04, Vol.39 (4), p.607-612
Main Authors: Javaran, Vahid Jalali, Shafeinia, Alireza, Javaran, Mokhtar Jalali, Gojani, Esmaeil Ghasemi, Mirzaee, Malihe
Format: Article
Language:English
Subjects:
Antibodies
Antibodies - immunology
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cucurbita - genetics
Cucurbita - metabolism
ELISA
Enzymatic activity
Enzymes
Flowers & plants
Gene Expression
Genes
Genetic recombination
Genetic Vectors
Humans
Life Sciences
Microbiology
Molecular chemistry
Molecular Weight
Mosaic Viruses
Original Research Paper
Plant tissues
Plants (organisms)
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Recombinant
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Tissue Plasminogen Activator - biosynthesis
Tissue Plasminogen Activator - genetics
Tissues
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Summary:Objective To use a transient expression system to express a truncated human tissue plasminogen activator (K2S) gene in cucurbit plants. Results The recombinant tissue plasminogen activator protein (K2S form) was expressed in active form in cucurbit plants. Its molecular weight was 43 kDa. The plant-derived rt-PA was determined using goat anti-rabbit antibody by western blotting. Among the infected lines, the highest expression of rt-PA was 62 ng/100 mg per leaf tissue as measured by ELISA. The enzymatic activity of the plant-derived rt-PA was 0.8 IU/ml. Conclusions The K25 form of rt-PA was expressed for the first time using the viral expression system. Plant-derived rt-PA showed similar potency to commercially-available PA.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-017-2285-6