An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores

This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes suffi...

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Bibliographic Details
Published in:Lab on a chip 2017-03, Vol.17 (6), p.1095-1103
Main Authors: Park, J, Salmi, M L, Wan Salim, W W A, Rademacher, A, Wickizer, B, Schooley, A, Benton, J, Cantero, A, Argote, P F, Ren, M, Zhang, M, Porterfield, D M, Ricco, A J, Roux, S J, Rickus, J L
Format: Article
Language:English
Subjects:
Automation, Laboratory
Calcium
Calcium - chemistry
Calcium - metabolism
Calibration
Chips
Devices
Equipment Design
Ferns
Ferns - chemistry
Ferns - cytology
Ferns - metabolism
Ferns - physiology
Gravitation
Lab-On-A-Chip Devices
Rotation
Space flight
Space Flight - instrumentation
Spores - chemistry
Spores - cytology
Spores - metabolism
Spores - physiology
Utilities
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Summary:This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions.
ISSN:1473-0197
1473-0189
DOI:10.1039/c6lc01370h