A rationally designed peptidomimetic biosensor for sialic acid on cell surfaces

We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to fluorescent streptavidin via biotin enabling the optical labeling of cells. This approach provides unique opportunities to detect Sia...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-05, Vol.51 (38), p.8112-8115
Main Authors: Chaudhary, Preeti Madhukar, Murthy, Raghavendra Vasudeva, Yadav, Rohan, Kikkeri, Raghavendra
Format: Article
Language:English
Subjects:
Animals
Arginine - chemistry
Backbone
Biosensing Techniques
Biosensors
Biotin - chemistry
Boronic Acids - chemistry
Cell Line, Tumor
Fluorescence
HeLa Cells
Humans
Labels
Marking
Mice
Molecular Structure
N-Acetylneuraminic Acid - analysis
NIH 3T3 Cells
Oligopeptides - chemical synthesis
Oligopeptides - chemistry
Peptides
Peptidomimetics
Streptavidin - chemistry
Surface Properties
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Summary:We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to fluorescent streptavidin via biotin enabling the optical labeling of cells. This approach provides unique opportunities to detect Sia composition on the cell surfaces and filopodia.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc01662b