Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

PEGylated gold nanoparticles (AuNPs) have an extended circulation time after intravenous injection in vivo and exhibit favorable properties for biosensing, diagnostic imaging, and cancer treatment. No impact of PEGylated AuNPs on the barrier forming properties of endothelial cells (ECs) has been rep...

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Bibliographic Details
Published in:Biomaterials science 2017-04, Vol.5 (4), p.707-717
Main Authors: Freese, C, Anspach, L, Deller, R C, Richards, S-J, Gibson, M I, Kirkpatrick, C J, Unger, R E
Format: Article
Language:English
Subjects:
Animals
Biomedical materials
Blood-brain barrier
Blood-Brain Barrier - drug effects
Blood-Brain Barrier - metabolism
Blood-Brain Barrier - pathology
Cells, Cultured
Endothelial cells
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Endothelial Cells - pathology
Erythrocytes
Gold
Gold - chemistry
Gold - metabolism
Gold - toxicity
Human Umbilical Vein Endothelial Cells
Humans
Imaging
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - metabolism
Nanoparticles - toxicity
Nanostructure
Particle Size
Polyethylene Glycols - chemistry
Polyethylene Glycols - metabolism
Polyethylene Glycols - toxicity
Swine
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Summary:PEGylated gold nanoparticles (AuNPs) have an extended circulation time after intravenous injection in vivo and exhibit favorable properties for biosensing, diagnostic imaging, and cancer treatment. No impact of PEGylated AuNPs on the barrier forming properties of endothelial cells (ECs) has been reported, but recent studies demonstrated that unexpected effects on erythrocytes are observed. Almost all studies to date have been with static-cultured ECs. Herein, ECs maintained under physiological cyclic stretch and flow conditions and used to generate a blood-brain barrier model were exposed to 20 nm PEGylated AuNPs. An evaluation of toxic effects, cell stress, the release profile of pro-inflammatory cytokines, and blood-brain barrier properties showed that even under physiological conditions no obvious effects of PEGylated AuNPs on ECs were observed. These findings suggest that 20 nm-sized, PEGylated AuNPs may be a useful tool for biomedical applications, as they do not affect the normal function of healthy ECs after entering the blood stream.
ISSN:2047-4830
2047-4849
DOI:10.1039/c6bm00853d