Novel approach to determine ghrelin analogs by liquid chromatography with mass spectrometry using a monolithic column

In our project, ghrelin analogs possessing enhanced stability and potential to significantly increase food intake were used. Three newly synthesized ghrelin analogs with fatty acid residues consisting of 8, 10, and 14 carbon atoms were studied. The main goal of this work was to develop a suitable an...

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Bibliographic Details
Published in:Journal of separation science 2017-03, Vol.40 (5), p.1032-1039
Main Authors: Zemenova, Jana, Sykora, David, Adamkova, Hana, Maletinska, Lenka, Elbert, Tomas, Marek, Ales, Blechova, Miroslava
Format: Article
Language:English
Subjects:
Analogs
Calibration
Carbon
Chromatography
Chromatography, Liquid
enzyme‐linked immunosorbent assay
Fatty acids
ghrelin
Ghrelin - analogs & derivatives
Kinetics
lipopeptides
Lipopeptides - blood
Liquid chromatography
liquid chromatography mass spectrometry
Mass spectrometry
Mathematical analysis
monolithic columns
Pharmacology
Reproducibility of Results
Separation
Spectroscopy
Stability
Stability analysis
Tandem Mass Spectrometry
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Summary:In our project, ghrelin analogs possessing enhanced stability and potential to significantly increase food intake were used. Three newly synthesized ghrelin analogs with fatty acid residues consisting of 8, 10, and 14 carbon atoms were studied. The main goal of this work was to develop a suitable analytical method for the determination of the stability of the novel ghrelin analogs in plasma. An appropriate liquid chromatography‐mass spectrometry method was developed and optimized. The results obtained were compared with the data measured by using a commercial enzyme‐linked immunosorbent assay kit, and a good correlation was found. A preparation strategy for plasma samples was optimized and consisted of simple dilution of the plasma samples followed by direct injection onto a very short monolithic column in combination with mass spectrometric detection. The developed analytical method was utilized for the determination of the stability of the prepared lipopeptides in plasma and for the quantification of the lipopeptides in a preliminary pharmacokinetic study. The feasibility of the developed separation method was clearly demonstrated. Accuracy and precision were within 80–120% and ±20% limits, respectively. Calibration curves were constructed in the range of 1–250 μg/mL.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201601141