Reversible DNA micro-patterning using the fluorous effect

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2017, Vol.53 (21), p.3094-3097
Main Authors: Flynn, Gabriella E, Withers, Jamie M, Macias, Gerard, Sperling, Justin R, Henry, Sarah L, Cooper, Jonathan M, Burley, Glenn A, Clark, Alasdair W
Format: Article
Language:English
Subjects:
Attachment
Binding
Deoxyribonucleic acid
DNA - chemistry
Efficiency
Gene sequencing
Hydrocarbons, Fluorinated - chemistry
Optical Imaging
Patterning
Regeneration
Solvents
Surface Properties
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Summary:We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc00288b