A New Determinant of Poor Outcome after Spontaneous Subarachnoid Hemorrhage; Blood pH and the Disruption of Glossopharyngeal Nerve-Carotid Body Network: First Experimental Study

• Published in: World neurosurgery 2017-08, Vol.104, p.330-338
• Main Authors: Kanat, Ayhan, Aydin, M. Dumlu, Bayram, Ednan, Kazdal, Hizir, Aydin, Nazan, Omeroglu, Muhammet, Altinkaynak, Konca, Kabalar, M. Esref, Yolas, Coskun, Ozturk, Cengiz, Kepoglu, Umit, Calik, Muhammet
• Format: Article
• Language: English
• Subjects: Acidosis; Acidosis - pathology; Acidosis - physiopathology; Animals; Blood Chemical Analysis; Carotid body; Carotid Body - pathology; Carotid Body - physiopathology; Disease Models, Animal; Glossopharyngeal nerve; Glossopharyngeal Nerve - pathology; Glossopharyngeal Nerve - physiopathology; Nerve Degeneration - pathology; Nerve Degeneration - physiopathology; Nerve Net - pathology; Nerve Net - physiopathology; Neurosurgery; Rabbits; Subarachnoid hemorrhage; Subarachnoid Hemorrhage - pathology; Subarachnoid Hemorrhage - physiopathology; Treatment Outcome
• ISSN: 1878-8750; 1878-8769
• DOI: 10.1016/j.wneu.2017.04.105

Abstract Objective The chemoreceptor network, consisting of the glossopharyngeal nerve and carotid body (GPN-CB), is essential for the regulation of blood pH. Its ischemic insuts following subarachnoid hemorrhage (SAH)which may contribute to develop acidosis has not been investigated. Materials and Methods Twent-three hybrid rabbits were used.They divided into three groups; Five (n=5) as control group, five (n=5) as SHAM group, the remaining 13 (n=13) as study group. 1cc serum saline, 1cc autolog arterial blood into the cisterna magna were injected; in SHAM and study group, respectively. Blood pH values of all annimals were recorded. After two weeks, animal were decapitated. The number of normal and degenerated neuron density ofCBs were counted by stereological methods,analysed statistically. Results Two of 13 rabbits died within the second week. The mean blood pH values were measured as 7.35±0.07 in control, 7.33±0.06 in SHAM, 7.29±0.05 in rabbits with slight acidisis (n=6) and 7.23±0.02 in in rabbits with prominent acidosis. In control groups, the average normal neuronal density of the CB was 6432±790/mm3 and degenerated neuron density was 11±3/mm3 while the degenerated neuronal density in CB was 35±8/mm3 in SHAM group, 1034±112/mm3 in slight acidis developed group (n=6; p