Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

• Published in: Biological & pharmaceutical bulletin 2017/05/01, Vol.40(5), pp.621-629
• Main Authors: Panthavee, Wanchai, Noda, Masafumi, Danshiitsoodol, Narandalai, Kumagai, Takanori, Sugiyama, Masanori
• Format: Article
• Language: English
• Subjects: Anti-inflammatory agents; anti-inflammatory substance; Bacteria; Biodegradation; Carbon sources; Culture Media; Degradation; DNA, Bacterial - genetics; exopolysaccharide; Exopolysaccharides; Fermentation; Fruit - microbiology; Fruits; Galactose; Galactose - chemistry; Glucose; Glucose - chemistry; Glycogen; Hyaluronic acid; Hyaluronoglucosaminidase - antagonists & inhibitors; Inflammation; Lactic acid; Lactic Acid - metabolism; Lactic acid bacteria; Lactobacillus - chemistry; Lactobacillus - genetics; Lactobacillus amylovorus; Mannose; Mannose - chemistry; Pediococcus - chemistry; Pediococcus - genetics; Pediococcus pentosaceus; Plants; Polysaccharides - chemistry; Polysaccharides - pharmacology; Sodium; Starch; Strains (organisms); Sugars - analysis; Temperature; Thailand; Thermophilic bacteria; thermophilic lactic acid bacteria; Yogurt
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b16-00856

In the present study, we have obtained two exopolysaccharide (EPS)-producing thermophilic lactic acid bacteria (LAB) that were isolated from tropical fruits of Thailand. The two strains, designated LY45 and PY45, were identified as Pediococcus pentosaceus and Lactobacillus amylovorus, respectively. Both plant-derived LAB strains, which produce neutral EPSs together with the acidic one, can grow vigorously at 45°C and even at 50°C. Hyaluronidase (EC 3.2.1.35), which catalyzes the degradation of hyaluronic acid, activates an inflammatory reaction. Interestingly, EPSs produced by the LY45 and PY45 strains were found to inhibit hyaluronidase activity at the same order of IC50 values as did sodium cromoglicate and dipotassium glycyrrhizinate, which are well-known as anti-inflammatory agents. The LY45-derived neutral EPS consists of glucose and mannose as monosaccharide components, whereas the acidic one contains mainly mannose, together with glucose and galactose. On the other hand, although Lactobacillus amylovorus PY45 also produces neutral and acidic EPSs, the main monosaccharide in both EPSs is mannose, and glucose is a minor component. Furthermore, the PY45 strain may be probiotically and industrially useful because the microorganism can utilize starch and glycogen as carbon sources.