Role of the complement‐lectin pathway in anaphylactoid reaction induced with lipopolysaccharide in mice

We show that Proteus vulgaris O25 (PO25) lipopolysaccharide (LPS) induced an anaphylactoid reaction not only in wild‐type and in lipid A non‐responding mice but also in recombinase‐activating gene‐2‐deficient (RAG‐2–/–) and in mast cell‐deficient (W/Wv) animals. Western blot analysis indicated that...

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Published in:European journal of immunology 2003-10, Vol.33 (10), p.2842-2852
Main Authors: Świerzko, Anna St, Cedzyński, Maciej, Kirikae, Teruo, Nakano, Masayasu, Klink, Magdalena, Kirikae, Fumiko, Ziółkowski, Andrzej, Vinogradov, Evgeny V., Kawakami, Masaya
Format: Article
Language:English
Subjects:
Anaphylactoid reaction
Anaphylaxis - etiology
Animals
Complement
Complement Activation
Fibrinogen - metabolism
Lectin pathway
Lipopolysaccharide
Lipopolysaccharides - toxicity
Mannan‐binding lectin
Mannose-Binding Lectin - physiology
Mannose-Binding Protein-Associated Serine Proteases
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Proteus vulgaris
Serine Endopeptidases - physiology
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Summary:We show that Proteus vulgaris O25 (PO25) lipopolysaccharide (LPS) induced an anaphylactoid reaction not only in wild‐type and in lipid A non‐responding mice but also in recombinase‐activating gene‐2‐deficient (RAG‐2–/–) and in mast cell‐deficient (W/Wv) animals. Western blot analysis indicated that PO25 LPS bound to Ra‐reactive factor (RaRF), the complex of mannan‐binding lectins (MBL) and MBL‐associated serine proteases. Binding of RaRF to PO25 LPS led to the activation of C4 component without participation of either C1 or Ig, via the lectin pathway. Relative concentration of RaRF and hemolytic activity in mouse serum decreased rapidly during the process of anaphylactoid reaction. A significant drop of MBL‐A, but not MBL‐C level was observed. Administrationwith antiserum to RaRF prevented animals from death as a consequence of the inhibition of interaction of RaRF with the carbohydrate target and complement activation. These results indicate that complement‐lectin pathway activation is responsible for the anaphylactoid reaction induced with LPS in muramyldipeptide‐primed mice. RaRF also activated fibrinogen in vitro suggesting the involvement of the coagulation system in the process investigated.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.200323949