The mobile element IS1207 of Brevibacterium lactofermentum ATCC21086: isolation and use in the construction of Tn5531, a versatile transposon for insertional mutagenesis of Corynebacterium glutamicum

IS1207 is the insertion most frequently found among the spontaneous mutations that abolish the activity of an Escherichia coli phage lambda cI gene integrated in the Corynebacterium Brevibacterium lactofermentum ATCC21086 genome. We examined the transposition of transposon-like structures composed o...

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Bibliographic Details
Published in:Journal of biotechnology 2003-09, Vol.104 (1-3), p.301-309
Main Authors: Bonamy, Celine, Labarre, Jean, Cazaubon, Laurent, Jacob, Christiane, Le Bohec, Françoise, Reyes, Oscar, Leblon, Gérard
Format: Article
Language:English
Subjects:
Brevibacterium - genetics
Brevibacterium lactofermentum
Corynebacterium - genetics
Corynebacterium glutamicum
DNA Transposable Elements - genetics
DNA, Bacterial - genetics
Gene Expression Regulation, Bacterial - genetics
Genetic Engineering - methods
Genetic Vectors - genetics
Mutagenesis, Insertional - methods
Sequence Analysis, DNA
transposon Tn5531
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Summary:IS1207 is the insertion most frequently found among the spontaneous mutations that abolish the activity of an Escherichia coli phage lambda cI gene integrated in the Corynebacterium Brevibacterium lactofermentum ATCC21086 genome. We examined the transposition of transposon-like structures composed of a selective kanamycin resistance gene (aph3), and one or two IS1207 sequences. One of these, the Tn5531 transposon, transposed efficiently in Corynebacterium glutamicum. A replicative and a non-replicative Tn5531 delivery vector were used in Tn5531 mutagenesis. As IS1207, transposon Tn5531 shows a high frequency of transposition and mutagenesis, and a low target specificity. These features make of Tn5531 an adequate choice for gene identification and gene tagging experiments.
ISSN:0168-1656
DOI:10.1016/S0168-1656(03)00150-0