Construction of ferrocene modified conducting polymer based amperometric urea biosensor

•An electrochemical urea biosensor has been constructed by electro-polymerizing SNS-Aniline on pencil graphite electrode.•The biosensor seems to be fast to respond and sensitive.•The electrode showed excellent performance in measuring of urea in blood and urine samples. Herein, an electrochemical ur...

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Bibliographic Details
Published in:Enzyme and microbial technology 2017-07, Vol.102, p.53-59
Main Authors: Dervisevic, Muamer, Dervisevic, Esma, Senel, Mehmet, Cevik, Emre, Yildiz, Huseyin Bekir, Camurlu, Pınar
Format: Article
Language:English
Subjects:
Amperometric biosensor
Aniline Compounds
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Electric Conductivity
Electrochemical Techniques - instrumentation
Electrochemical Techniques - methods
Enzymes, Immobilized
Ferrocene
Ferrous Compounds
Humans
Metallocenes
Polymers
Pyrroles
SNS aniline
Thiophenes
Urea
Urea - analysis
Urea - blood
Urea - urine
Urease
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Summary:•An electrochemical urea biosensor has been constructed by electro-polymerizing SNS-Aniline on pencil graphite electrode.•The biosensor seems to be fast to respond and sensitive.•The electrode showed excellent performance in measuring of urea in blood and urine samples. Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor’s amperometric response was linear in the range of 0.1–8.5mM with the sensitivity of 0.54μA/mM, detection limit of 12μM, and short response time of 2s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insignificant interference.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2017.04.002