Effect of oxidant demand on the release and degradation of microcystin-LR from Microcystis aeruginosa during oxidation

• Published in: Chemosphere (Oxford) 2017-08, Vol.181, p.562-568
• Main Authors: Zhang, Haiting, Dan, Yongbo, Adams, Craig D., Shi, Honglan, Ma, Yinfa, Eichholz, Todd
• Format: Article
• Language: English
• Subjects: Cell lysis; Chlorination; Chlorine - pharmacology; Cyanobacteria; Halogenation; Manganese Compounds - pharmacology; Microcystin-LR; Microcystins - metabolism; Microcystis - metabolism; Microcystis aeruginosa; Oxidants - metabolism; Oxidants - pharmacology; Oxidation-Reduction; Oxides - pharmacology; Permanganate; Toxin release rate; Water Microbiology; Water Purification - methods
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.04.120

In this research, the release and degradation of intracellular microcystin-LR (MC-LR) due to oxidation of Microcystis aeruginosa (M. aeruginosa) was examined kinetically. Brief exposure to free chlorine with no measureable oxidant exposure was demonstrated to be sufficient to induce rapid release of intracellular MC-LR from M. aeruginosa. Thus, in a water treatment plant, there is currently no level of prechlorination that can be assumed to be safe, since very low preoxidation prior to filtration and no measureable free chlorine residual may still observe the release and buildup of extracellular MC-LR. Higher chlorine dosages resulting in a measureable exposure or CT (concentration times contact time) cause more rapid release and oxidation of the intracellular toxins. Further, the rate of release of MC-LR with intermediate oxidant dosages were shown to be initially rapid, but then slowed to a lower release rate due to an as yet undetermined mechanism. While free chlorine was reactive with the extracellular MC-LR, the monochloramine resulting from the consumption of the free chlorine by ammonia was not. Consideration of the ammonia concentration and the chlorine dosage relative to the chlorination breakpoint dosages is important for utilities assessing the impact of prechlorination of water containing cyanobacteria. MC-LR, once released, was rapidly oxidized by permanganate resulting in only negligible buildup of extracellular toxins. •MC-LR can be rapidly released from M. aeruginosa with very low and non-measureable chlorine and permanganate exposures.•There may not be sufficiently low preoxidation level that can be assumed to not release toxins in a water treatment plant.•The oxidant-induced rate of MC-LR release from M. aeruginosa was initially very rapid and then decreases with time.•Oxidation and release kinetics were modeled for MC-LR release.•Ammonia exerts a rapid demand on chlorine and plays an important role on MC-LR release.