New HLA-DRB107 allele identified from microlymphocytotoxicity/SSP discrepancies

• Published in: Tissue antigens 2002-12, Vol.60 (6), p.539-541
• Main Authors: Donvito, B., Perrier, P., Tonye Libyh, M., Tabary, T., Philbert, F., Reveil, B., Cohen, J.H.M.
• Format: Article
• Language: English
• Subjects: Alleles; Base Sequence; Cytotoxicity Tests, Immunologic; Exons; Histocompatibility Testing; HLA-DR Antigens - chemistry; HLA-DR Antigens - genetics; HLA-DR Antigens - immunology; HLA-DRB1 Chains; HLA-DRB10706; Humans; microlymphocytotoxicity; molecular biology HLA typing; Molecular Sequence Data; Protein Structure, Secondary
• ISSN: 0001-2815; 1399-0039
• DOI: 10.1034/j.1399-0039.2002.600611.x

: A HLA–DRB1*07 variant allele has been identified in a cadaver kidney donor. Serological typing using monoclonal antibodies detected HLA–DR4 and HLA–DR7. HLA class II DNA typing using sequence‐specific primer (PCR‐SSP) polymerase chain reaction only detected DRB1*04, while sequence‐specific oligonucleotide (PCR‐SSO) polymerase chain reaction confirmed the presence of both DRB1*04 and DRB1*07 alleles, although two extra reactions were also found. Exon 2 of the HLA‐DRB1*07 was isolated using allele‐specific PCR, then cloned and sequenced. Four mutations, at positions 170 (T → C), 171 (C → T), 174 (C → G), and 179 (C → A), were observed. These mutations changed codons 57 and 60 (V → A; S → Y, respectively). This amino acid sequence at position 56–61 is only found in DRB1*0811.