Differential accumulation of Xanthomonas campestris pv. campestris proteins during the interaction with the host plant: Contributions of an in vivo system

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot, a highly destructive disease that affects all brassicas. This work aimed to study the interaction Xcc–Brassica oleracea using an in vivo system in an attempt to identify proteins involved in pathogenicity. We used label‐fr...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2017-06, Vol.17 (12), p.n/a
Main Authors: Santos, Cristiane, Maximiano, Mariana R., Ribeiro, Daiane G., Oliveira‐Neto, Osmundo B., Murad, André M., Franco, Octávio L., Mehta, Angela
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Black rot
Black rot disease
Brassica
Brassica - growth & development
Brassica - metabolism
Brassica - microbiology
Cell culture
Gene expression
Host plants
Host-Pathogen Interactions
In vivo methods and tests
Label‐free proteomics
Pathogenicity
Pathogens
Plant Diseases - microbiology
Plant extracts
Plant Leaves - growth & development
Plant Leaves - metabolism
Plant Leaves - microbiology
Plant–pathogen interaction
Proteins
Proteome - metabolism
Two dimensional analysis
Virulence Factors - genetics
Virulence Factors - metabolism
Xanthomonas campestris
Xanthomonas campestris - pathogenicity
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Summary:Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot, a highly destructive disease that affects all brassicas. This work aimed to study the interaction Xcc–Brassica oleracea using an in vivo system in an attempt to identify proteins involved in pathogenicity. We used label‐free shotgun 2D‐nanoUPLC/MSE to analyze Xcc proteins in three conditions: in the interaction with susceptible (REK) and resistant (REU) plants and in culture medium (control condition). A model of Xcc–susceptible host interaction is proposed and shows that Xcc increases the abundance of several crucial proteins for infection and cell protection. In this study, we also confirmed the differential expression by qPCR analysis of selected genes. This is the first report showing a large‐scale identification of proteins in an in vivo host plant condition. Considering that most studies involving phytopathogens are in vitro (growth in culture medium or in plant extract), this work contributes with relevant information related to the plant–pathogen interaction in planta.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201700086