Method development for the analysis of resinous materials with MALDI‐FT‐ICR‐MS: novel internal standards and a new matrix material for negative ion mode

• Published in: Journal of mass spectrometry. 2017-09, Vol.52 (9), p.603-617
• Main Authors: Teearu, A., Vahur, S., Rodima, T., Herodes, K., Bonrath, W., Netscher, T., Tshepelevitsh, S., Trummal, A., Lõkov, M., Leito, I.
• Format: Article
• Language: English
• Subjects: 2‐aminoacridine; Anions; Calibration; Cations; Composition; Cultural heritage; Cultural resources; Cyclotron resonance; Desorption; Dihydroxybenzoic acid; Fluorine; Fourier transforms; High resolution; internal calibration; Ionization; Ions; Lasers; MALDI‐FT‐ICR‐MS; Mass spectra; Mass spectrometry; Mass spectroscopy; Materials selection; Methods; phosphazene; resinous materials; Resins; Spectra; sulphonimide
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.3943

Matrix‐assisted laser desorption/ionization (MALDI) is a mass spectrometry (MS) ionization technique suitable for a wide variety of sample types including highly complex ones such as natural resinous materials. Coupled with Fourier transform ion cyclotron resonance (FT‐ICR) mass analyser, which provides mass spectra with high resolution and accuracy, the method gives a wealth of information about the composition of the sample. One of the key aspects in MALDI‐MS is the right choice of matrix compound. We have previously demonstrated that 2,5‐dihydroxybenzoic acid is suitable for the positive ion mode analysis of resinous samples. However, 2,5‐dihydroxybenzoic acid was found to be unsuitable for the analysis of these samples in the negative ion mode. The second problem addressed was the limited choice of calibration standards offering a flexible selection of m/z values under m/z 1000. This study presents a modified MALDI‐FT‐ICR‐MS method for the analysis of resinous materials, which incorporates a novel matrix compound, 2‐aminoacridine for the negative ion mode analysis and extends the selection of internal standards with m/z