2-Amino- N-(2-furylmethyl)propanamide as a novel alanylglycine equivalent synthesized by bacilysin synthetase

Bacilysin is a dipeptide antibiotic composed of l-alanine and l-anticapsin. We recently reported partial purification and characterization of the bacilysin synthetase. In the present study, l-anticapsin in the in vitro reaction mixture was replaced by furfurylamine, norephedrine, glycine, and benzyl...

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Bibliographic Details
Published in:Enzyme and microbial technology 2003-10, Vol.33 (5), p.725-728
Main Authors: Tüzün, Işıl, Karataş, Ayten Y., Şeşenoğlu, Özge, Demir, Ayhan S., Özcengiz, Gülay
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacilysin
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Nonribosomal biosynthesis
Peptide synthetases
Peptides
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Summary:Bacilysin is a dipeptide antibiotic composed of l-alanine and l-anticapsin. We recently reported partial purification and characterization of the bacilysin synthetase. In the present study, l-anticapsin in the in vitro reaction mixture was replaced by furfurylamine, norephedrine, glycine, and benzylamine, respectively. The enzymatic biosynthesis of novel structures was checked by TLC analyses of reaction mixture extracts and any positive TLC result was verified by GC–MS analysis. The enzyme was shown to link furfurylamine to l-alanine, hence synthesizing 2-amino- N-(2-furylmethyl)propanamide, but did not act on the other substrates tested to replace anticapsin.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(03)00209-6