Genome-wide Expression Profiling (with Focus on the Galectin Network) in Tumor, Transition Zone and Normal Tissue of Head and Neck Cancer: Marked Differences Between Individual Patients and the Site of Specimen Origin

Expression profiling was performed to delineate and characterize the impact of malignancy by comparing tissues from three sites of head and neck cancer of each patient, also determining interindividual variability. Genome-wide analysis was carried out covering the expression of 25,832 genes with qua...

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Bibliographic Details
Published in:Anticancer research 2017-05, Vol.37 (5), p.2275-2288
Main Authors: Zivicova, Veronika, Broz, Petr, Fik, Zdenek, Mifkova, Alzbeta, Plzak, Jan, Cada, Zdenek, Kaltner, Herbert, Kucerova, Jana Fialova, Gabius, Hans-Joachim, Smetana, Jr, Karel
Format: Article
Language:English
Subjects:
Aged
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - metabolism
Cytokines - genetics
Epithelium - metabolism
Female
Galectins - genetics
Galectins - metabolism
Gene Expression Profiling
Genome, Human
Humans
Keratins - genetics
Keratins - metabolism
Male
Middle Aged
Oropharyngeal Neoplasms - genetics
Oropharyngeal Neoplasms - metabolism
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Summary:Expression profiling was performed to delineate and characterize the impact of malignancy by comparing tissues from three sites of head and neck cancer of each patient, also determining interindividual variability. Genome-wide analysis was carried out covering the expression of 25,832 genes with quantification for each site of seven patients with tonsillar or oropharyngeal squamous cell carcinoma. Immunohistochemical analysis was performed for adhesion/growth-regulatory galectins, three pro-inflammatory chemo- and cytokines and keratins. Up- and down-regulation was found for 281 (tumor vs. normal) and 276 genes (transition zone vs. normal), respectively. The profile of the transition zone had its own features, with similarity to the tumor. Galectins were affected in a network manner, with differential regulation and interindividual variability between patients, also true for keratins and the chemo- and cytokines. These results underline special features at each site of specimen origin as well as the importance of analyzing galectins as a network and of defining the expression status of the individual patient prior to reaching clinically relevant conclusions.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.11565