Expression of hsa-miR-9 and MYC Copy Number Variation in Hereditary Diffuse Gastric Cancer

• Published in: Anticancer research 2017-05, Vol.37 (5), p.2401-2406
• Main Authors: DA Silva Oliveira, Kelly Cristina, Bona, Amanda Braga, DA Silva, Fernanda Jardim, Pinheiro, Thayanne Macedo, DI Felipe Avila Alcantara, Diego, Lamarao, Leticia Martins, Moreira-Nunes, Caroline Aquino, Assumpcao, Paulo Pimentel, Burbano, Rommel Rodriguez, Calcagno, Danielle Queiroz
• Format: Article
• Language: English
• Subjects: Adult; Aged; Cadherins - genetics; DNA Copy Number Variations; Female; Humans; Male; MicroRNAs - genetics; Middle Aged; Proto-Oncogene Proteins c-myc - genetics; Stomach Neoplasms - genetics
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.11579

Approximately, 15-50% of families affected by hereditary diffuse gastric cancer (HDGC) exhibit CDH1 germline mutations. CDH1 gene encodes E-cadherin, protein essential to the cell-cell contact of gastric epithelium. Studies have shown that hsa-miR-9 participates in this protein downregulation. Moreover, MYC is responsible for the transcription of hsa-miR-9-3. In the present study, hsa-miR-9 expression and MYC copy number variation were investigated to elucidate the hsa-miR-9 role in HDGC. Tumor samples were obtained from nine individuals with HDGC history belonging to four Brazilian families. Then, relative quantification of hsa-miR-9 expression and MYC gene copy number variation analysis were performed by real-time PCR. In all the samples, an overexpression of hsa-miR-9 and an increased MYC copy number (≥3 copies) were observed. hsa-miR-9 acts as an oncomiR in HDGC. In addition, we suggest that hsa-miR-9 acts as second event in individuals with HDGC carrying CDH1 gene germinline mutations.