Evaluation of OSMOCELLS, a new semi‐automatic device for osmotic fragility assessment

Summary Introduction The osmotic fragility (OF) test was a central test for the diagnosis of hereditary red blood cell (RBC) disorders (mostly hereditary spherocytosis (HS), but thalassaemia as well). Nowadays although the traditional multitubes method has lost a prominent place, many laboratories s...

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Bibliographic Details
Published in:International journal of laboratory hematology 2017-10, Vol.39 (5), p.521-527
Main Authors: Gérard, D., Fattet, A‐J., Brakta, C., Phulpin, A., Steschenko, D., Lesesve, J‐F., Perrin, J.
Format: Article
Language:English
Subjects:
Adult
Anticoagulants
Anticoagulants - pharmacology
Automation, Laboratory
Blood diseases
Blood donors
Dialysis
Erythrocytes
Female
haemolysis
Hematologic Tests - instrumentation
Hematologic Tests - methods
Hematologic Tests - standards
Hereditary spherocytosis
Humans
Laboratories
Male
Middle Aged
osmocells
osmotic fragility
Osmotic Fragility - drug effects
red blood cell disorders
Reference Values
Reproducibility of Results
Spherocytosis
Spherocytosis, Hereditary
Standardization
Thalassemia
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Summary:Summary Introduction The osmotic fragility (OF) test was a central test for the diagnosis of hereditary red blood cell (RBC) disorders (mostly hereditary spherocytosis (HS), but thalassaemia as well). Nowadays although the traditional multitubes method has lost a prominent place, many laboratories still perform such a laboured test, despite the lack of standardization. In fact, the evaluation of OF may offer an inexpensive screening for RBC disorders. We present a new semi‐automatic device, allowing the continuous recording of OF, by an updated dialysis method. Methods Repeatability, stability over time, influence of the anticoagulant were evaluated among a population of healthy blood donors. The test was then performed among patients presenting inherited RBC disorders (HS or haemoglobinopathies) where OF is typically altered. Results Repeatability was excellent; the parameters were greatly influenced by the nature of the anticoagulant and interestingly appeared stable for 48 h. Patients with RBC disorders displayed the expected profile in regard with their disease: patients with HS all presented an increased OF while patients with haemoglobinopathy displayed resistant profiles. Conclusion The device offers a substantial improvement in terms of standardization and consistency of the results and may offer a considerable gain for general laboratories.
ISSN:1751-5521
1751-553X
DOI:10.1111/ijlh.12683