Engineered Bacillus pumilus laccase-like multi-copper oxidase for enhanced oxidation of the lignin model compound guaiacol

Abstract Laccases and laccase-like multi-copper oxidases (LMCOs) are versatile and robust biocatalysts applied in a variety of oxidative processes, and various studies have attempted to improve their catalytic activity. Here we report the engineering of a bacterial LMCO for enhanced oxidation of the...

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Bibliographic Details
Published in:Protein engineering, design and selection design and selection, 2017-06, Vol.30 (6), p.449-453
Main Authors: Ihssen, Julian, Jankowska, Dagmara, Ramsauer, Thomas, Reiss, Renate, Luchsinger, Ronny, Wiesli, Luzia, Schubert, Mark, Thöny-Meyer, Linda, Faccio, Greta
Format: Article
Language:English
Subjects:
Bacillus pumilus - enzymology
Bacillus pumilus - genetics
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Guaiacol - chemistry
Guaiacol - metabolism
Laccase - chemistry
Laccase - genetics
Laccase - metabolism
Lignin
Models, Molecular
Oxidation-Reduction
Oxidoreductases - chemistry
Oxidoreductases - genetics
Oxidoreductases - metabolism
Protein Engineering
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
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Summary:Abstract Laccases and laccase-like multi-copper oxidases (LMCOs) are versatile and robust biocatalysts applied in a variety of oxidative processes, and various studies have attempted to improve their catalytic activity. Here we report the engineering of a bacterial LMCO for enhanced oxidation of the lignin-related compound guaiacol by a combination of structure-guided mutagenesis and DNA shuffling. Mutant L9 showed a 1.39 mM Km for guaiacol and a 2.5-fold increase in turnover rate (kcat/Km = 2.85·104 M−1s−1).
ISSN:1741-0126
1741-0134
DOI:10.1093/protein/gzx026