Hydrophobic Interaction Chromatography for Bottom-Up Proteomics Analysis of Single Proteins and Protein Complexes

Hydrophobic interaction chromatography (HIC) is a robust standard analytical method to purify proteins while preserving their biological activity. It is widely used to study post-translational modifications of proteins and drug–protein interactions. In the current manuscript we employed HIC to separ...

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Published in:Journal of proteome research 2017-06, Vol.16 (6), p.2318-2323
Main Authors: Rackiewicz, Michal, Große-Hovest, Ludger, Alpert, Andrew J, Zarei, Mostafa, Dengjel, Jörn
Format: Article
Language:English
Subjects:
Cell Line
Chromatography, Liquid
Chromatography, Reverse-Phase - methods
Humans
Hydrophobic and Hydrophilic Interactions
Multiprotein Complexes - analysis
Proteins - analysis
Proteomics - methods
Tandem Mass Spectrometry
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cited_by cdi_FETCH-LOGICAL-a398t-e4bb5be3629d5bc26247b2855166811753f0c89eac4991623ca3eea4e13c022d3
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container_issue 6
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container_title Journal of proteome research
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creator Rackiewicz, Michal
Große-Hovest, Ludger
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Zarei, Mostafa
Dengjel, Jörn
description Hydrophobic interaction chromatography (HIC) is a robust standard analytical method to purify proteins while preserving their biological activity. It is widely used to study post-translational modifications of proteins and drug–protein interactions. In the current manuscript we employed HIC to separate proteins, followed by bottom-up LC–MS/MS experiments. We used this approach to fractionate antibody species followed by comprehensive peptide mapping as well as to study protein complexes in human cells. HIC–reversed-phase chromatography (RPC)–mass spectrometry (MS) is a powerful alternative to fractionate proteins for bottom-up proteomics experiments making use of their distinct hydrophobic properties.
doi_str_mv 10.1021/acs.jproteome.7b00015
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Cell Line
Chromatography, Liquid
Chromatography, Reverse-Phase - methods
Humans
Hydrophobic and Hydrophilic Interactions
Multiprotein Complexes - analysis
Proteins - analysis
Proteomics - methods
Tandem Mass Spectrometry
title Hydrophobic Interaction Chromatography for Bottom-Up Proteomics Analysis of Single Proteins and Protein Complexes
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