Mathematical model of polymerase chain reaction with temperature-dependent parameters

The course of the real-time polymerase chain reaction (PCR) is determined by the temperature dependence of the kinetics of the component reactions, particularly the DNA strand hybridization. To investigate the effect of thermal processes on the reaction behavior, a mathematical model in which the va...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2017, Vol.472 (1), p.77-80
Main Authors: Sochivko, D. G., Fedorov, A. A., Alekseev, Y. I., Kurochkin, V. E., Dubina, M. V.
Format: Article
Language:English
Subjects:
Base Pairing
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
DNA - chemistry
Life Sciences
Mathematical models
Models, Theoretical
Molecular Biology
Nucleic Acid Denaturation
Polymerase chain reaction
Polymerase Chain Reaction - methods
Taq Polymerase - chemistry
Taq Polymerase - metabolism
Temperature
Temperature effects
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Summary:The course of the real-time polymerase chain reaction (PCR) is determined by the temperature dependence of the kinetics of the component reactions, particularly the DNA strand hybridization. To investigate the effect of thermal processes on the reaction behavior, a mathematical model in which the variable rate constant of dissociation of “primer–single strand” complexes depends on temperature was proposed. The reaction medium temperature, which depends on time, was also introduced into the model. The proposed model of real-time PCR makes it possible to analyze different aspects of the reaction, which are important for the development of instruments and reagents for PCR.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672917010240