New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein

Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide...

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Bibliographic Details
Published in:Virus genes 2017-06, Vol.53 (3), p.434-445
Main Authors: Han, Sang-Hyuk, Park, Jong-Seo, Han, Jae-Yeong, Gong, Jun-Su, Park, Chan-Hwan, Kim, Jung-Kyu, Seo, Eun-Young, Domier, Leslie L., Hammond, John, Lim, Hyoun-Sub
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Substitution
Biomedical and Life Sciences
Biomedicine
Bromoviridae
Capsicum - virology
Capsicum annuum
Cloning, Molecular
Cucumovirus - genetics
DNA, Complementary - genetics
Genome, Viral - genetics
Medical Microbiology
Nicotiana - virology
Nicotiana benthamiana
Phylogeny
Plant Diseases - virology
Plant Sciences
Promoter Regions, Genetic
Republic of Korea
RNA Viruses - genetics
Tobamovirus - genetics
Tobamovirus - isolation & purification
Tobamovirus - pathogenicity
Viral Proteins - genetics
Virology
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Summary:Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76. In addition, we generated infectious clones of S-47 and J-76, and T7 promoter driven transcripts of each inoculated to Nicotiana benthamiana produced very severe symptoms, whereas only mild symptoms developed in Capsicum annuum . Gene silencing suppressor function of 126 kDa and cytoskeleton-connected plasmodesmata localization of movement protein of S-47 and J-76 showed no difference between isolates, whereas 126 kDa of J-76 clearly formed intracellular aggregates not observed with S-47 126 kDa protein. Differences between these isolates in 126/183 kDa-related functions including subcellular localization suggest that differential interactions with host proteins may affect symptom development in C. annuum .
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-017-1432-4