Transient and stable CHO expression, purification and characterization of novel hetero‐dimeric bispecific IgG antibodies

• Published in: Biotechnology progress 2017-03, Vol.33 (2), p.469-477
• Main Authors: Rajendra, Yashas, Peery, Robert B., Hougland, Maria D., Barnard, Gavin C., Wu, Xiufeng, Fitchett, Jonathan R., Bacica, Michael, Demarest, Stephen J.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - isolation & purification; bispecific antibodies; Cell Proliferation - physiology; Chinese hamster ovary; CHO; CHO Cells; Cloning, Molecular - methods; Cricetulus; Immunoglobulin G - immunology; Mass spectrometry; Protein Engineering - methods; quad vector; Recombinant Proteins - immunology; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Transfection - methods
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1002/btpr.2414

IgG bispecific antibodies (BsAbs) represent one of the preferred formats for bispecific antibody therapeutics due to their native‐like IgG properties and their monovalent binding to each target. Most reported studies utilized transient expression in HEK293 cells to produce BsAbs. However, the expression of biotherapeutic molecules using stable CHO cell lines is commonly used for biopharmaceutical manufacturing. Unfortunately, limited information is available in the scientific literature on the expression of BsAbs in CHO cell lines. In this study we describe an alternative approach to express the multiple components of IgG BsAbs using a single plasmid vector (quad vector). This single plasmid vector contains both heavy chain genes and both light chain genes required for the expression and assembly of the IgG BsAb, along with a selectable marker. We expressed, purified, and characterized four different IgG BsAbs or “hetero‐mAbs” using transient CHO expression and stable CHO minipools. Transient CHO titers ranged from 90 to 160 mg/L. Stable CHO titers ranged from 0.4 to 2.3 g/L. Following a simple Protein A purification step, the percentage of correctly paired BsAbs ranged from 74% to 98% as determined by mass spectrometry. We also found that information generated from transient CHO expression was similar to information generated using stable CHO minipools. In conclusion, the quad vector approach represents a simple, but effective, alternative approach for the generation of IgG BsAbs in both transient CHO and stable CHO expression systems. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:469–477, 2017