Real-Time PCR Identification of Six Malassezia Species

Lipophilic yeast Malassezia species is widely found on the skin surface of humans and other animals. This fungus can cause pityriasis versicolor, Malassezia folliculitis, and seborrheic dermatitis. Still now, there is a problem with species identification of Malassezia with conventional methods. We...

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Published in:Current microbiology 2017-06, Vol.74 (6), p.671-677
Main Authors: Ilahi, Amin, Hadrich, Inès, Neji, Sourour, Trabelsi, Houaida, Makni, Fattouma, Ayadi, Ali
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biotechnology
Cats
Cattle - microbiology
Dermatitis
Dogs
Domestic animals
Equidae - microbiology
Fungi
Goats - microbiology
Humans
Life Sciences
Malassezia
Malassezia - classification
Malassezia - genetics
Malassezia - isolation & purification
Microbiology
Molecular Typing - methods
Mycological Typing Techniques - methods
Nucleic Acid Denaturation - genetics
Polymerase chain reaction
Probes
Rabbits
Real-Time Polymerase Chain Reaction - methods
RNA, Ribosomal - genetics
Skin - microbiology
Skin diseases
Tinea Versicolor - microbiology
Yeast
Yeasts
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description Lipophilic yeast Malassezia species is widely found on the skin surface of humans and other animals. This fungus can cause pityriasis versicolor, Malassezia folliculitis, and seborrheic dermatitis. Still now, there is a problem with species identification of Malassezia with conventional methods. We developed a real-time polymerase chain reaction (PCR) assay with multiple hybridization probes for detecting M. globosa, M. furfur, M. restricta, M. sympodialis, M. slooffiae , and M. pachydermatis . The amplification curves and specific melting peaks of the probes hybridized with real-time PCR product were used for species identifications. The assay was further evaluated on 120 samples which were performed by swabbing from 60 domestic animals (23 goats, 10 dogs, 15 cows, 3 cats, 8 rabbits, and 1 donkey) and in 70 human samples (28 patients with pityriasis versicolor, 17 breeders, and 25 control group). Fifteen M. pachydermatis were identified from animals. From human, 61 isolates were identified as M. globosa (28), M. furfur (15), M. restricta (6), M. sympodialis (8), M. slooffiae (2), and M. pachydermatis (2). Eight cases of co-detection from 6 patients and 2 breeders were revealed. Our findings show that the assay was highly effective in identifying Malassezia species. The application of multiplex real-time PCR provides a sensitive and rapid identification system for Malassezia species, which may be applied in further epidemiological surveys from clinical samples.
doi_str_mv 10.1007/s00284-017-1237-7
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source Springer Nature
subjects Animals
Biomedical and Life Sciences
Biotechnology
Cats
Cattle - microbiology
Dermatitis
Dogs
Domestic animals
Equidae - microbiology
Fungi
Goats - microbiology
Humans
Life Sciences
Malassezia
Malassezia - classification
Malassezia - genetics
Malassezia - isolation & purification
Microbiology
Molecular Typing - methods
Mycological Typing Techniques - methods
Nucleic Acid Denaturation - genetics
Polymerase chain reaction
Probes
Rabbits
Real-Time Polymerase Chain Reaction - methods
RNA, Ribosomal - genetics
Skin - microbiology
Skin diseases
Tinea Versicolor - microbiology
Yeast
Yeasts
title Real-Time PCR Identification of Six Malassezia Species
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