Simultaneous Determination of 20 Anti-Obesity Drugs Illegally Added in Weight-loss Functional Foods by Matrix Solid Phase Dispersion-High Performance Liquid Chromatography Tandem Mass Spectrometry

• Published in: Fēnxī huàxué 2014-08, Vol.42 (8), p.1161-1170
• Main Authors: MA, Wei, CHENG, Li, ZHANG, Lan-Wei, ZHANG, Ying-Chun, WANG, Hai-Bo, JIAO, Yue-Hua, DAI, Han-Hui, TANG, Ying-Zhang
• Format: Article
• Language: chi ; eng
• ISSN: 0253-3820
• DOI: 10.11895/j.issn.0253-3820.140113

An analytical method based on high performance liquid chromatography tandem mass spectrometry has been developed for the simultaneous determination of 20 anti-obesity drugs (fenfluramine, phenylpropanolamine, sibutramine, sertraline, rimonabant, bupropion, citalopram, fluoxetine, benfluorex, topiramate, zonisamide, caffeine, phenolphthalein, emodin, indapamide, bumetanide, torasemide, triamterene, orlistat, phenformin) that were extracted from various weight-loss functional foods by ethanol-acetone(7:3, V/V) and purified by primary secondary amine (PSA) and octadecyltrimethoxysilane (ODS) under ultrasonication. The analysis was carried out on HPLC-MS/MS by electrospray ionization using multiple reaction monitoring after the chromatographic separation on Waters Atlantis T3 (3 mu m, 150 mm x 2.1 mm) column. Identification was achieved by the retention time and the ion ratio, quantification was done by the external standard method. The limits of detection for the appetite suppressants were 0.05-3.0 mg/kg. The mean recoveries at the three spiked levels were 67.1% - 101.4%, with the intra-day precision less than 10% and the inter-day precision less than 15%. The method is reliable, accurate, reproducible and suitable for the determination of the anti-obesity drugs in different weight-loss functional foods.