DNA chip based sensor for amperometric detection of infectious pathogens

• Published in: International journal of biological macromolecules 2017-10, Vol.103, p.355-359
• Main Authors: Singh, Swati, Kaushal, Ankur, Khare, Shashi, Kumar, Ashok
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - methods; DNA chip; Electrochemistry; Limit of Detection; Oligonucleotide Array Sequence Analysis; PAMAM dendrimer; Pathogens; Rheumatic heart disease; Streptococcus pyogenes; Streptococcus pyogenes - genetics; Streptococcus pyogenes - isolation & purification
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.05.041

[Display omitted] •DNA chip based sensor for amperometric detection of infectious pathogens.•Sensor takes only 30min for detection of pathogen.•The LOD of the sensor was 130fg/6μL and the linear range was 10−3–10−1ng/6μL.•The sensor is highly specific for S. pyogenes and has potential for point-of-care diagnosis. Several infectious pathogens are found in human whose detection is essential for rapid cure of diseases. The most commonly found pathogen in human is Streptococcus pyogenes which leads to a wide range of infections from mild pharyngitis to rheumatic heart disease. An ultrasensitive DNA chip based sensor was developed for quick identification of pathogen S. pyogenes from patient throat swab samples. The amperometric response was measured after hybridization of specific probe with single stranded genomic DNA (ssG-DNA) from the patient samples. The DNA chip was characterized by FTIR, SEM and validated with suspected patient real samples. The sensitivity of the DNA chip based sensor was found 951.34(μA/cm2)/ng DNA and lower limit of detection (LOD) was 130fg/6μL samples. The DNA chip based sensor is highly specific and takes only 30min for identification of specific pathogen.