Spring bud growth depends on sugar delivery by xylem and water recirculation by phloem Münch flow in Juglans regia

• Published in: Planta 2017-09, Vol.246 (3), p.495-508
• Main Authors: Tixier, Aude, Sperling, Or, Orozco, Jessica, Lampinen, Bruce, Roxas, Adele Amico, Saa, Sebastian, Earles, J. Mason, Zwieniecki, Maciej A.
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; Buds; Carbohydrates; Ecology; Forestry; Girdling; Juglans - growth & development; Juglans - metabolism; Juglans - physiology; Life Sciences; Limbs; Moisture content; ORIGINAL ARTICLE; Perfusion; Phloem; Phloem - physiology; Photosynthesis; Plant Sciences; Plant Shoots - growth & development; Plant Shoots - metabolism; Plant Shoots - physiology; Shoots; Stems; Sugar; Sugars - metabolism; Translocation; Transpiration; Transport; Trees - growth & development; Trees - metabolism; Trees - physiology; Water - metabolism; Water content; Xylem; Xylem - physiology
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-017-2707-7

During the spring, carbohydrate demand of developing buds often exceeds locally available storage, thus requiring the translocation of sugars from distant locations like limbs, stems and roots. Both the phloem and xylem have the capacity for such long-distance transport, but their functional contribution is unclear. To address this ambiguity, the spatial and temporal dynamics of carbohydrate availability in extension shoots of Juglans regia L. were analyzed. A significant loss of extension shoot carbohydrates in remote locations was observed while carbohydrate availability near the buds remained unaffected. This pattern of depletion of carbohydrate reserves supports the notion of long-distance translocation. Girdling and dye perfusion experiments were performed to assess the role of phloem and xylem in the transport of carbohydrate and water towards the buds. Girdling caused a decrease in nonstructural carbohydrate concentration above the point of girdling and an unexpected concurrent increase in water content associated with impeded xylem transport. Based on experimental observations and modeling, we propose a novel mechanism for maintenance of spring carbohydrate translocation in trees where xylem transports carbohydrates and this transport is maintained with the recirculation of water by phloem Münch flow. Phloem Münch flow acts as a pump for generating water flux in xylem and allows for transport and mobilization of sugars from distal locations prior to leaves photosynthetic independence and in the absence of transpiration.