Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2015-08, Vol.6 (8), p.4508-4512
Main Authors: Tran, Mai N, Rarig, Robert-André F, Chenoweth, David M
Format: Article
Language:English
Subjects:
Biology
Dyes
Fluid flow
Fluorescent dyes
Monitoring
Organelles
Synthesis
Tracking
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Summary:Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application of a new class of large Stokes shift fluorescent dyes that are water-soluble, cell permeable, non-cytotoxic, and lysosome-specific. Additionally, we demonstrate temporally controlled sequential photoactivation of individual cells in close spatial proximity.
ISSN:2041-6520
2041-6539
DOI:10.1039/c5sc01601k