Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury

• Published in: Chinese journal of integrative medicine 2018-04, Vol.24 (4), p.272-277
• Main Authors: Zhang, Shu-kun, Zhuo, Yu-zhen, Li, Cai-xia, Yang, Lei, Gao, Hong-wei, Wang, Xi-mo
• Format: Article
• Language: English
• Subjects: Animals; CD18 Antigens - metabolism; Cell Adhesion - drug effects; Docosahexaenoic Acids - administration & dosage; Docosahexaenoic Acids - pharmacology; Docosahexaenoic Acids - therapeutic use; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Injections; Intercellular Adhesion Molecule-1 - metabolism; Leukocytes - drug effects; Leukocytes - metabolism; Leukocytes - pathology; Lung - drug effects; Lung - enzymology; Lung - pathology; Lung Injury - blood; Lung Injury - complications; Lung Injury - drug therapy; Male; Medicine; Medicine & Public Health; Mice, Inbred C57BL; Original Article; Peroxidase - metabolism; Sepsis - blood; Sepsis - complications; Sepsis - drug therapy; Survival Analysis
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-017-2959-x

Objective To investigate the effect of combined application of Xuebijing Injection (血必净注 射液, XBJ) and resolvin D1 (RvD1) on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. Methods The cecal ligation and puncture (CLP) method was used to develop a mouse sepsis model. Specific pathogen free male C57BL/6 mice were randomly divided into 5 groups ( n =20 each): sham, CLP, CLP+XBJ, CLP+RvD1 and CLP+XBJ+RvD1. After surgery, mice in the CLP+XBJ, CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight), RvD1 (10 ng/g body weight), and their combination (the same dose of XBJ and RvD1), respectively. In each group, 12 mice were used to observe 1-week survival rate, while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18, lung tissues were harvested for observing pathological changes, and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1). Results Compared with the CLP group, the histopathological damage of the lung tissues was mitigated, MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups ( P