Voltammetric determination of reduced glutathione using poly(thionine) as a mediator in the presence of Fenton-type reaction

An indirect and sensitive electrochemical method for the determination of reduced glutathione (GSH) was developed by using poly(thionine) (PTH) as a mediator in the presence of Fenton-type reaction on the electrode surface in this work. Cyclic voltammetry and the adsorption of Cu2+ were employed in...

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Bibliographic Details
Published in:Talanta (Oxford) 2017-08, Vol.170, p.399-405
Main Authors: Liu, Baihui, Ma, Chao, Li, Yi, Kou, Yanxia, Lu, Jiajia, Jiang, Xiangmei, Tan, Liang
Format: Article
Language:English
Subjects:
Adsorption
Copper - chemistry
Electrochemical Techniques - methods
Electrodes
Electroreduction
Fenton-type reaction
Glutathione - analysis
Glutathione - urine
Humans
Hydrogen Peroxide - chemistry
Inhibition action
Iron - chemistry
Limit of Detection
Oxidation-Reduction
Phenothiazines - chemistry
Poly(thionine)
Polymers - chemistry
Reduced glutathione
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Summary:An indirect and sensitive electrochemical method for the determination of reduced glutathione (GSH) was developed by using poly(thionine) (PTH) as a mediator in the presence of Fenton-type reaction on the electrode surface in this work. Cyclic voltammetry and the adsorption of Cu2+ were employed in sequence to fabricate a Cu-PTH modified electrode, which was characterized by scanning electron microscopy, XPS and electrochemical measurements. Hydroxyl radicals that were derived from the Fenton-type reaction between Cu2+ and H2O2 could effectively oxidize PTH, leading to the great enhancement of the cathodic peak current of the dye polymer in the cyclic voltammetric scan. The electroreduction of PTH on the electrode surface was found to be inhibited in the presence of GSH. Under the optimized conditions, the cathodic peak current change was found to be proportional to the logarithm of the GSH concentration from 10nM to 1mM with a detection limit of 2.5nM estimated at a signal-to-noise ratio of 3. The proposed electrochemical sensor was successfully employed for the determination of GSH in real samples with satisfactory results. [Display omitted] •The product of Fenton-type reaction on GCE surface effectively oxidized PTH.•PTH in the oxidation state acted as a mediator.•A sensitive method for the determination of reduced glutathione was developed.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2017.04.019