Structure and gene cluster of the O-antigen of Escherichia albertii O1 resembling the O-antigen of Pseudomonas aeruginosa O5

• Published in: Carbohydrate research 2017-06, Vol.446-447, p.28-31
• Main Authors: Zheng, Han, Shashkov, Alexander S., Xiong, Yanwen, Naumenko, Olesya I., Wang, Hong, Senchenkova, Sof'ya N., Wang, Jianping, Knirel, Yuriy A.
• Format: Article
• Language: English
• Subjects: 2,3-Diacetamido-2,3-dideoxyhexuronic acid; Bacterial polysaccharide structure; Carbohydrate Sequence; Escherichia - chemistry; Escherichia - genetics; Escherichia albertii; Lipopolysaccharide; Multigene Family; O Antigens - chemistry; O Antigens - genetics; O-antigen gene cluster; O-specific polysaccharide; Pseudomonas aeruginosa; Pseudomonas aeruginosa - chemistry
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2017.04.024

The O-specific polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Escherichia albertii serotype O1 strain SP20140089 and studied by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy. The following structure was established for the trisaccharide repeating unit of the O-polysaccharide: →4)-β-d-ManpNAc3NAcA-(1 → 4)-β-d-GlcpNAm3NAcA-(1 → 3)-α-d-GlcpNAc-(1→ where ManNAc3NAcA and GlcNAm3NAcA indicate 2,3-diacetamido-2,3-dideoxymannuronic acid and 2-acetimidoylamino-3-acetamido-2,3-dideoxyglucuronic acid, respectively. While showing some similarity with O-polysaccharide structures of a group of Pseudomonas aeruginosa serotypes (O2, O5, O16, O18, and O20), that of E. albertii O1 is unique among known bacterial polysaccharide structures. The gene cluster for biosynthesis of the O1-antigen was sequenced and functions of the genes were predicted by comparison with sequences in the available databases, including those involved in the synthesis of nucleotide precursors of 2,3-diamino-2,3-dideoxyhexuronic acid derivatives in P. aeruginosa O5. [Display omitted] •An O-specific polysaccharide were isolated from Escherichia albertii O1.•The polysaccharide is enriched in derivatives of diamino uronic acids.•Structures of the polysaccharide was established by NMR spectroscopy.•Gene cluster for biosynthesis of the polysaccharide was found to match the structure.