Enhancement of rotator cuff tendon–bone healing with fibroblast growth factor 2 impregnated in gelatin hydrogel sheets in a rabbit model

• Published in: Journal of shoulder and elbow surgery 2017-10, Vol.26 (10), p.1708-1717
• Main Authors: Tokunaga, Takuya, MD, PhD, Karasugi, Tatsuki, MD, PhD, Arimura, Hitoshi, MD, Yonemitsu, Ryuji, MD, Sakamoto, Hidetoshi, Dr Eng, Ide, Junji, MD, PhD, Mizuta, Hiroshi, MD, PhD
• Format: Article
• Language: English
• Subjects: Animals; Biomechanical Phenomena; biomechanics; Drug Carriers; FGF-2; Fibroblast Growth Factor 2 - pharmacology; Gelatin; gelatin hydrogel sheet; histology; Hydrogel, Polyethylene Glycol Dimethacrylate; Models, Animal; Orthopedics; rabbit model; Rabbits; rotator cuff; Rotator Cuff - drug effects; Rotator Cuff - pathology; Rotator Cuff - surgery; tendon–bone healing; Wound Healing - drug effects; X-Ray Microtomography
• ISSN: 1058-2746; 1532-6500
• DOI: 10.1016/j.jse.2017.03.020

Background Application of fibroblast growth factor 2 (FGF-2) may improve the healing response after rotator cuff (RC) surgical repair. This study aimed to determine whether FGF-2–impregnated gelatin hydrogel sheet (GHS) incorporation into the bony trough on the greater tuberosity facilitates healing after RC surgical repair in rabbits. Methods We assigned 120 adult male Japanese white rabbits treated with unilateral surgery for supraspinatus tendon repair into the following groups: suture-only group (suture); suture and GHS with phosphate-buffered saline (carrier); suture and GHS with 3 µg of FGF-2 (F3); and suture and GHS with 30 µg of FGF-2 (F30). The effect of FGF-2 was assessed using histologic, biomechanical, and microcomputed tomography evaluations at 2, 6, and 12 weeks. Results At 12 weeks, loose fibrovascular tissues emerged at the repair site in the suture and carrier groups and dense tendon-like tissues in the F3 and F30 groups, which demonstrated significantly higher ultimate load-to-failure and stress-to-failure at 12 weeks than that in the suture and carrier groups. Microcomputed tomography imaging showed ectopic calcification formation in some specimens from each group. Appearances or frequencies were similar among groups. The histologic and biomechanical effects of FGF-2 on RC healing were obvious at ≥6 weeks postoperatively. Conclusion FGF-2-impregnated GHS incorporation into the bony trough on the greater tuberosity before RC surgical repair is feasible and results in histologic and biomechanical improvements during RC healing in rabbits. No detrimental effect on ectopic calcification was observed.