The zebrafish embryo as a model to quantify early inflammatory cell responses to biomaterials

• Published in: Journal of biomedical materials research. Part A 2017-09, Vol.105 (9), p.2522-2532
• Main Authors: Zhang, Xiaolin, Stockhammer, Oliver. W., de Boer, Leonie, Vischer, Norbert. O. E, Spaink, Herman. P., Grijpma, Dirk. W., Zaat, Sebastian. A. J.
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Biocompatible Materials - adverse effects; biomaterial microspheres; Biomaterials; Biomedical materials; Cell Communication; Cell Movement; Complexity; Disease Models, Animal; early inflammatory cell responses; Embryo, Nonmammalian - pathology; Embryos; Fluorescence; Fluorescence microscopy; in vivo imaging; In vivo methods and tests; Infiltration; Inflammation; Inflammation - pathology; Leukocyte migration; Leukocytes; Leukocytes (neutrophilic); Macrophages; Macrophages - pathology; material properties; Microscopy; Microspheres; Neutrophil Infiltration; Neutrophils; Polyesters - adverse effects; Polystyrene; Polystyrene resins; Polystyrenes - adverse effects; Proteins; Real time; Recruitment; Surgical implants; T cell receptors; Zebrafish; Zebrafish - embryology; zebrafish embryo
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.36110

To rapidly assess early inflammatory cell responses provoked by biomaterials in the full complexity of the living organism, we developed a zebrafish embryo model which allows real time analysis of these responses to biomaterial microspheres. Fluorescently labeled microspheres with different properties were injected into embryos of selected transgenic zebrafish lines expressing distinct fluorescent proteins in their neutrophils and macrophages. Recruitment of leukocytes and their interactions with microspheres were monitored using fluorescence microscopy. We developed a novel method using ImageJ and the plugin ObjectJ project file “Zebrafish‐Immunotest” for rapid and semi‐automated fluorescence quantification of the cellular responses. In the embryo model we observed an ordered inflammatory cell response to polystyrene and poly (ε‐caprolactone) microspheres, similar to that described for mammalian animal models. The responses were characterized by an early infiltration of neutrophils followed by macrophages, and subsequent differentially timed migration of these cells away from the microspheres. The size of microspheres (10 and 15 µm) did not influence the cellular responses. Poly (ε‐caprolactone) microspheres provoked a stronger infiltration of neutrophils and macrophages than polystyrene microspheres did. Our study shows the potential usefulness of zebrafish embryos for in vivo evaluation of biomaterial‐associated inflammatory cell responses. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2522–2532, 2017.