Adaptive conditions and safety of the application of Penicillium frequentans as a biocontrol agent on stone fruit

• Published in: International journal of food microbiology 2017-08, Vol.254, p.25-35
• Main Authors: Guijarro, Belén, Larena, Inmaculada, Melgarejo, Paloma, De Cal, Antonieta
• Format: Article
• Language: English
• Subjects: Animal health; Animals; Antifungal activity; Antifungal agents; Ascomycota - growth & development; Biological control; Biological Control Agents - adverse effects; Biological Control Agents - pharmacology; Brown rot; Citrinin; Citrinin - isolation & purification; Climatic conditions; Darkness; Data analysis; Data processing; Environmental impact; Environmental monitoring; Europe; Food safety; Food Safety - methods; Fruit - microbiology; Fruits; Fungi; Fungicides; Growth rate; High-performance liquid chromatography; In vitro methods and tests; Incubation; Light intensity; Liquid chromatography; Luminous intensity; Microorganisms; Monilinia spp; Mycelia; Mycelium - growth & development; Mycotoxins; Mycotoxins - isolation & purification; Ochratoxin A; Ochratoxins - isolation & purification; Patulin; Patulin - isolation & purification; Penicillic acid; Penicillic Acid - isolation & purification; Penicillin; Penicillium - metabolism; pH effects; Phylogeny; Product safety; Prunus persica - microbiology; Risk assessment; Spectrophotometry; Sporulation; Stone fruit safety; Temperature effects; Water activity
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2017.05.004

Penicillium frequentans (Pf909) reduces brown rot caused by Monilinia spp. in stone fruit. The registration of a microbial biocontrol agent in Europe requires information on the risks and safety of a biological product. This study focused on the impact of the physical environment on Pf909 survival and growth, Pf909 mycotoxin production on fruit surface, and the Pf909 resistance to commercial antifungal compounds used in animal and human medicine. The effect of temperature (4 to 37°C), water activity (0.999 to 0.900), pH (3 to 11), light intensity (0, 90 and 180lm) and photoperiod (0/24, 12/12, 16/8, 24/0; light/dark) on mycelial growth and sporulation of Pf909 were monitored for 10days in vitro on culture medium. Antifungal activity of antifungal compounds on mycelial growth of Pf909 was also measured by a quantitative micro spectrophotometric assay after 72h of incubation. The presence or absence of four non-volatile mycotoxins (patulin, penicillic acid, ochratoxin A and citrinin) on nectarine surfaces treated with Pf909 was determined by HPLC. Growth rate was significantly influenced by water activity, temperature and light exposure conditions. Pf909 showed maximum growth and sporulation at 22°C and 25°C, in wet conditions (0.999 water activity), with a pH5.6 to 9, and in darkness or a short light photoperiod. Our results showed all antifungal compounds used reduced significantly Pf909 mycelial growth at tested commercial doses. HPLC data analysis showed that 7days after biofungicide (Pf909) application there were no mycotoxin products on the surface of nectarine. Finally, no phylogenetic relationship has been shown among Pf909 and other species of Penicillium that produce mycotoxins. In conclusion, from an ecological point of view, Pf909 would establish and survive actively over a broad range of climatic conditions. The probability of risks to human and animal health is considered very low. •Pf909 is an effective and safe BCA against brown rot caused by Monilinia spp.•Pf909 applications when temperatures are warm•Pf909 does not produce mycotoxins.•Pf909 is phylogenetically unrelated to mycotoxin producers.•Pf909 is sensitive to antifungal compounds used in human disease treatment.