Autophagy-related gene microarray and bioinformatics analysis for ischemic stroke detection

Ischemic stroke (IS) is characterized by high morbidity and poor prognosis. However, the mechanisms of IS induced injury are still poorly understood. The main aim of this study is to explore the role of autophagy in IS. Ten pairs of whole blood samples of IS patients and matched controls were includ...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2017-07, Vol.489 (1), p.48-55
Main Authors: Guo, Yinsheng, Ma, Yue, Zhang, Yanwei, Zhou, Li, Huang, Suli, Wen, Ying, Zou, Fei, Cheng, Jinquan
Format: Article
Language:English
Subjects:
Aged
Autophagy
Autophagy - genetics
Brain Ischemia - genetics
Brain Ischemia - pathology
Case-Control Studies
Case-control study
Computational Biology
Female
Gene Expression Profiling
Gene microarray
GO analysis
Humans
Ischemic stroke
Male
Middle Aged
Pathway analysis
Polymerase Chain Reaction
Stroke - genetics
Stroke - pathology
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Summary:Ischemic stroke (IS) is characterized by high morbidity and poor prognosis. However, the mechanisms of IS induced injury are still poorly understood. The main aim of this study is to explore the role of autophagy in IS. Ten pairs of whole blood samples of IS patients and matched controls were included to select differential expressed genes (DE genes) by autophagy-related functional gene microarray analysis. And then, one hundred and fifty pairs of whole blood samples of IS patients and matched controls were included to validate the DE genes. Moreover, Gene Ontology (GO) analyses and Pathway analyses were also performed based on the DE gene results. Our results indicated that the co-regulation of autophagy and apoptosis took part in IS-induced injuries, and mitochondrial autophagy and apoptosis played a crucial role in this process. Furthermore, lysosome, protein kinase and endopeptidase also participated in IS. These findings clarified the role of mitochondrial autophagy and apoptosis in ischemic stroke and provided more important biomarkers for the prevention diagnosis and therapeutic implications in IS. •Blood samples were used to identify biomarkers of IS with autophagy microarray.•Results of GO analysis showed that the prominent cellular component was lysosome.•Protein kinase and endopeptidase were the prominent molecular functions.•Apoptosis pathway was the most enriched pathway.•Mito-autophagy and apoptosis play a crucial role in IS-induced injury.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2017.05.099